Bulk shear viscosities of endogenous and exogenous lung surfactants.
Bulk shear viscosities of endogenous and exogenous lung surfactants.
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内源性和外源性肺表面活性剂的体积剪切粘度。
DOI：
10.1152/ajplung.00199.2001
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Notter,RobertH
中科院分区：
文献类型：
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作者：
King,DavidM;Wang,Zhengdong;Palmer,HarveyJ;Holm,BruceA;Notter,RobertH
体积剪切粘度用锥板显微粘度计测量，作为灌洗小牛肺表面活性剂（LS），Exosurf，Infasurf，Survanta和合成脂质混合物分散在生理盐水中的浓度，剪切速率和温度的函数。粘度随磷脂浓度的所有表面活性剂，但其幅度和剪切依赖性变化很大，在不同的制剂。Exosurf和合成磷脂的盐水分散体具有仅几厘泊（cp）的低粘度，并表现出最小的剪切依赖性。LS、Infasurf、Survanta和含有棕榈酸和tripalmitin的脂质混合物具有较大的非牛顿粘度，其随着剪切速率降低而增加。在35 mg磷脂/ml和37°C下，剪切速率为77 s−1时粘度值为52.3 cp（Survanta）、31.1 cp（LS）和25 cp（Infasurf），770 s−1时粘度值为16.9 cp（Survanta）、10.1 cp（LS）和6.6 cp（Infasurf）。在25 mg磷脂/ml和37°C下，77 s− 1时的粘度值为28.8 cp（Survanta）、4.7 cp（LS）和12.5 cp（Infasurf）。在固定剪切速率下，与37°C相比，LS和Infasurf在23°C下的粘度显著降低，但Survanta的粘度增加。钙（5 mM）大大降低了Survanta和Infasurf在37°C下的粘度。合成混合物的研究表明，磷脂/载脂蛋白的相互作用是重要的肺衍生的表面活性剂的流变学和棕榈酸和tripalmitin有助于增加粘度的Survanta。临床外源性表面活性剂的粘性行为可能影响其在肺中的递送和分布，并且随组成、浓度、温度、离子环境和物理制剂而显著变化。
Bulk shear viscosities were measured with a cone and plate microviscometer as a function of concentration, shear rate, and temperature for lavaged calf lung surfactant (LS), Exosurf, Infasurf, Survanta, and synthetic lipid mixtures dispersed in normal saline. Viscosity increased with phospholipid concentration for all surfactants, but its magnitude and shear dependence varied widely among the different preparations. Saline dispersions of Exosurf and synthetic phospholipids had low viscosities of only a few centipoise (cp) and exhibited minimal shear dependence. LS, Infasurf, Survanta, and lipid mixtures containing palmitic acid and tripalmitin had larger non-Newtonian viscosities that increased as shear rate decreased. At 35 mg of phospholipid/ml and 37°C, viscosity values were 52.3 cp (Survanta), 31.1 cp (LS), and 25 cp (Infasurf) at a shear rate of 77 s−1and 16.9 cp (Survanta), 10.1 cp (LS), and 6.6 cp (Infasurf) at 770 s−1. At 25 mg of phospholipid/ml and 37°C, viscosity values at 77 s−1were 28.8 cp (Survanta), 4.7 cp (LS), and 12.5 cp (Infasurf). At fixed shear rate, viscosity was substantially decreased at 23°C compared with 37°C for LS and Infasurf but was increased for Survanta. Calcium (5 mM) greatly reduced the viscosity of both Survanta and Infasurf at 37°C. Studies on synthetic mixtures indicated that phospholipid/apoprotein interactions were important in the rheology of lung-derived surfactants and that palmitic acid and tripalmitin contributed to the increased viscosity of Survanta. The viscous behavior of clinical exogenous surfactants potentially influences their delivery and distribution in lungs and varies significantly with composition, concentration, temperature, ionic environment, and physical formulation.