Senescence marker protein-30 as a novel antiaging molecule
Senescence marker protein-30 as a novel antiaging molecule
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DOI：
10.1196/annals.1297.062
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发表时间：
2004-01-01
期刊：
影响因子：
--
通讯作者：
Maruyama, N
中科院分区：
文献类型：
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作者：
Feng, DY;Kondo, Y;Maruyama, N
衰老标记蛋白 30 (SMP30) 由 299 个氨基酸组成，分子量约为 32-34 kDa，电荷等电点 (pI) 4.9。来自不同动物物种的氨基酸比对揭示了高度保守的结构。 SMP30具有水解沙林、梭曼和塔崩的酶活性，这些化学物质被称为致命的有毒神经化学物质。我们使用 DFP 作为底物分析了 SMP30 的有机磷酸酶活性。在镁离子存在的情况下，这种 DFPase 活性以剂量依赖性方式显现。我们研究了 SMP30 的细胞内定位。它位于细胞质和细胞核中。为了确认细胞核中是否存在 SMP30，我们从分离的培养肝细胞中制备了细胞核和细胞质提取物。 Western blotting 显示两种提取物中均检测到 SMP30。由于四氯化碳会降低表达，因此可以推测该表达受到随衰老而增加的氧化应激的调节。
Senescence marker protein-30 (SMP30), composed of 299 amino acids, has an approximate molecular mass of 32-34 kDa and has a pI 4.9 in charge. The amino acid alignment from various animal species revealed a highly conserved structure. SMP30 has an enzyme activity hydrolyzing sarin, soman, and tabun, known as lethal toxic nerve chemicals. We analyzed the organophosphatase activity of SMP30 using DFP as a substrate. This DFPase activity is revealed in a dose-dependent manner in the presence of magnesium ions. We investigated the intracellular localization of SMP30. It is localized in both the cytoplasm and nucleus. To confirm the presence of SMP30 in the nucleus, we prepared nuclear and cytoplasmic extracts from isolated cultured hepatocytes. Western blotting showed that SMP30 was detected in both extracts. Because the expression is reduced by carbon tetrachloride, one can speculate that the expression is modulated by oxidative stress increased with aging.