DNA and low titer, helper-free, recombinant AAV prime-boost vaccination for cytomegalovirus induces an immune response to CMV-pp65 and CMV-IE1 in transgenic HLA A*0201 mice.
DNA and low titer, helper-free, recombinant AAV prime-boost vaccination for cytomegalovirus induces an immune response to CMV-pp65 and CMV-IE1 in transgenic HLA A*0201 mice.
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DOI：
10.1016/j.vaccine.2004.06.048
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发表时间：
2004-12
期刊：
影响因子：
5.5
通讯作者：
G. Gallez-Hawkins;Xiuli Li;Anne E. Franck;Lia Thao;S. Lacey;D. Diamond;J. Zaia
中科院分区：
文献类型：
--
作者：
G. Gallez-Hawkins;Xiuli Li;Anne E. Franck;Lia Thao;S. Lacey;D. Diamond;J. Zaia
初免-加强免疫方案允许使用低滴度、无辅助因子的 rAAV-pp65mII 和 rAAV-IE1 病毒来引发针对两种重要的巨细胞病毒 (CMV) 抗原的特异性体液和细胞反应：立即早期 1 (IE-1) 和 pp65 蛋白。使用 DNA 疫苗引发和 rAAV 加强同时免疫两种 CMV 蛋白，诱导抗体 (Ab) 反应、具有细胞毒功能的 CD8 淋巴细胞，并使用四聚体技术检测人 HLA A*0201 限制性同源肽表位的结合。
A prime-boost immunization regimen allowed the use of low titer, helper-free rAAV-pp65mII and rAAV-IE1 virus to elicit specific humoral and cellular responses to two important cytomegalovirus (CMV) antigens: the immediate-early 1 (IE-1) and pp65 proteins. Simultaneous immunization of both CMV proteins, using DNA vaccine priming followed by rAAV boost, induced antibody (Ab) response, CD8 lymphocytes with cytotoxic function, and detectible binding of the cognate peptide epitopes for human HLA A*0201 restriction using tetramer technology.