Catalase-dependent peroxidative metabolism in the alveolar macrophage during phagocytosis.
Catalase-dependent peroxidative metabolism in the alveolar macrophage during phagocytosis.
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吞噬过程中肺泡巨噬细胞中过氧化氢酶依赖性过氧化代谢。
DOI：
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发表时间：
1970
影响因子：
15.9
通讯作者：
J. Field
中科院分区：
文献类型：
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作者：
J. Gee;C. Vassallo;P. Bell;J. Kaskin;R. Basford;J. Field
兔肺泡巨噬细胞（AM）中存在过氧化代谢的证据已从以下观察中获得：(a)过氧化氢酶高浓度存在；(b)以愈创木酚为底物无法检测过氧化物酶活性；(c)氨基三唑对AM过氧化氢酶的不可逆抑制作为H(2)O(2)的检测系统，并显示吞噬后细胞内H(2)O(2)增加；(d)甲酸氧化，过氧化氢酶依赖性过氧化的标志，发生在静息AM中，并通过吞噬作用增加；(c) AM透析液中H(2)O(2)积累的测量表明，在吞噬过程中增加了两倍；(f)氨基三唑减少了O(2)的利用和标记葡萄糖和丙酮酸的（14）CO(2)的产生。由此得出结论，虽然过氧化氢酶依赖的H(2)O(2)代谢不是颗粒进入所必需的，但这一途径代表了颗粒进入AM所刺激的代谢途径之一。
EVIDENCE FOR THE PRESENCE OF PEROXIDATIVE METABOLISM IN RABBIT ALVEOLAR MACROPHAGES (AM) HAS BEEN OBTAINED FROM THE FOLLOWING OBSERVATIONS: (a) catalase is present in high concentrations; (b) peroxidase activity could not be detected employing guaiacol as substrate; (c) the irreversible inhibition of AM catalase by aminotriazole served as a detection system for H(2)O(2) and demonstrated increased intracellular H(2)O(2) after phagocytosis; (d) formate oxidation, a marker of catalase-dependent peroxidations, occurs in resting AM and is increased by phagocytosis; (c) measurements of H(2)O(2) accumulation in a dialysate of AM demonstrated twofold increase during phagocytosis; and (f) aminotriazole diminishes O(2) utilization and (14)CO(2) production from labelled glucose and pyruvate. It is concluded that, while catalase-dependent H(2)O(2) metabolism is not essential for particle entry, this pathway represents one of the metabolic pathways stimulated by particle entry in the AM.