CDC14 of Saccharomyces cerevisiae. Cloning, sequence analysis, and transcription during the cell cycle.
CDC14 of Saccharomyces cerevisiae. Cloning, sequence analysis, and transcription during the cell cycle.
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DOI：
10.1016/s0021-9258(19)49907-9
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发表时间：
1992-06
期刊：
影响因子：
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通讯作者：
J. Wan;H. Xu;M. Grunstein
中科院分区：
文献类型：
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作者：
J. Wan;H. Xu;M. Grunstein
我们克隆、定位和测序了酿酒酵母CDC14基因，并对其在细胞周期中的转录进行了研究。Cdc14位于VI号染色体一对3.5kb的XhoI-XbaI片段中。该DNA序列显示一个开放阅读框架，可编码一个423个氨基酸的多肽。通过ProSite、GenBank和EMBL数据库的蛋白质序列比较，我们可以确定在编码的CDC14蛋白质中有一个保守的蛋白质酪氨酸磷酸酶活性部位，从153个氨基酸开始。Disrupt表明CDC14是一个必不可少的基因。CDC14转录本的水平似乎受到细胞周期的弱调控，并且具有周期性，滞后于组蛋白HTB1 mRNA积累水平约15分钟。DNA序列分析发现，CDC14启动子中有一个区域与组蛋白H_2A1-H_2B1(HTA1-HTB1)基因对的启动子的细胞周期调节区有惊人的相似之处(21个碱基对中有15个相同)。细胞周期调控序列负责组蛋白HTA1-HTB1信息的周期性积累和羟基尿素敏感性。然而，与组蛋白mRNA不同的是，组蛋白mRNA在羟基脲停滞时受到抑制，CDC14 mRNA似乎不受影响。这表明CDC14和组蛋白基因在细胞周期中受不同机制的调控。此外，超螺旋密度测量表明CDC14不参与核小体组装。
We have cloned, mapped and sequenced the complete CDC14 gene of Saccharomyces cerevisiae and characterized its transcription during the cell cycle. CDC14 was found within a 3.5-kilobase pair XhoI-XbaI fragment of chromosome VI. The DNA sequence reveals an open reading frame capable of encoding a 423-amino acid polypeptide. Protein sequence comparisons through the Prosite, GenBank and EMBL databases allowed us to identify a conserved protein tyrosine phosphatase active site in the encoded CDC14 protein beginning at amino acid 153. Disruption demonstrates that CDC14 is an essential gene. The level of the CDC14 transcript appears to be weakly cell cycle-regulated and has a periodicity which lags approximately 15 min behind histone HTB1 mRNA accumulation levels. DNA sequence analysis has identified a region within the CDC14 promoter which bears a striking resemblance (15 out of 21 base pairs identity) to the cell cycle regulation region of the promoter of the histone H2A1-H2B1 (HTA1-HTB1) gene pair. The cell cycle regulation sequence is responsible for the periodic accumulation and hydroxyurea sensitivity of the histone HTA1-HTB1 message. However, unlike histone mRNA, which is repressed upon hydroxyurea arrest, CDC14 mRNA appears to be unaffected. This suggests that CDC14 and histone genes are regulated by different mechanisms during the cell cycle. Furthermore, superhelical density measurements suggest that CDC14 is not involved in nucleosome assembly.