The PLCO Biorepository: Creating, Maintaining, and Administering a Unique Biospecimen Resource.
The PLCO Biorepository: Creating, Maintaining, and Administering a Unique Biospecimen Resource.
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DOI：
10.2174/1574887110666150730121429
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发表时间：
2015
影响因子：
1.9
通讯作者：
Zhu C
中科院分区：
文献类型：
--
作者：
Carrick DM;Black A;Gohagan JK;Khan A;Pettit K;Williams C;Yu K;Yurgalevitch S;Huang WY;Zhu C
在基于人群的研究中纳入生物标本是理解疾病病因、识别生物标志物和制定预防和治疗策略的一个组成部分。前列腺、肺、结直肠和卵巢（PLCO）癌症筛查试验从参与者中收集、处理和储存生物标本，以创建标本生物储存库，作为广泛研究社区的有用资源。PLCO在六轮筛选中从同意的筛选组参与者中采集血液样本，并从同意的对照组参与者中采集口腔样本。此外，还为双臂参与者收集了选定癌症部位的福尔马林固定石蜡包埋肿瘤组织标本。在多个时间点（即系列样本）和癌症诊断前收集生物样本，再加上丰富的流行病学和筛查数据，使PLCO收集的生物样本成为一种独特的宝贵资源。因此，通过严格的科学审查程序，并在负责制定和实施生物标本使用政策的指导委员会的指导下，向研究者授予PLCO生物储存库的使用权。在这里，我们描述了生物标本的采集，处理，储存，申请和分发，以及在PLCO数据管理的程序。我们还提供了生物标本如何用于推进癌症研究的例子，并描述了相关的经验教训，以帮助通知希望添加或修改生物标本采集的队列。
Inclusion of biospecimens in population-based studies is an integral part of understanding disease etiology, identifying biomarkers and developing prevention and treatment strategies. The Prostate, Lung, Colorectal, and Ovarian (PLCO) cancer screening trial collected, processed and stored biospecimens from participants to create a biorepository of specimens which serves as a useful resource for a broad research community. PLCO collected blood samples from consented screening arm participants at six screening rounds and a buccal sample from consented control arm participants. In addition, formalin-fixed paraffin embedded tumor tissue specimens were collected for participants in both arms for selected cancer sites. Collection of biospecimens at multiple timepoints (i.e. serial samples) and prior to cancer diagnosis, paired with rich epidemiologic and screening data, makes the PLCO collection of biospecimens a uniquely valuable resource. As such, access to the PLCO biorepository is granted to investigators by a rigorous scientific review process and guided by a steering committee which is responsible for developing and implementing the biospecimen use policies. Here, we describe the procedures for biospecimen collection, processing, storage, requisition, and distribution, as well as data management employed in PLCO. We also provide examples of how the biospecimens have been used to advance cancer research and describe relevant lessons learned to help inform cohorts wishing to add or modify biospecimen collection.