Time-lapse viscoelastic imaging of living fibroblasts using force modulation mode in AFM.
Time-lapse viscoelastic imaging of living fibroblasts using force modulation mode in AFM.
复制标题
使用 AFM 力调制模式对活成纤维细胞进行延时粘弹性成像。
DOI：
10.1093/oxfordjournals.jmicro.a023831
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
T. Sambongi
中科院分区：
文献类型：
--
作者：
H. Haga;M. Nagayama;K. Kawabata;E. Ito;T. Ushiki;T. Sambongi
使用原子力显微镜中的力调制模式，我们已经成功地捕获活的小鼠成纤维细胞（NIH3T3）在生理条件下几个小时的延时粘弹性图像，而不损害成纤维细胞。每10分钟拍摄一次的延时地形图图像中观察到片状伪足的伸长和水泡的肿胀。在600 Hz的频率下，相应的粘弹性反应被可视化为连续图像。细胞体的较硬部分相当稳定，超过1 h未显示形态学变化。这可能是由于肌动蛋白网络的过度凝聚，硬化细胞皮层，降低细胞骨架活性。细胞体的核部分似乎比周边区域的粘性稍低。
Using the force modulation mode in atomic force microscopy, we have succeeded in capturing time-lapse viscoelastic images of living mouse fibroblasts (NIH3T3) for several hours in a physiological condition without damaging the fibroblasts. Elongation of the lamellipodia and swelling of blebs were observed in time-lapse topographic images, which were taken every 10 min. The corresponding viscoelastic responses at a frequency of 600 Hz were visualized as consecutive images. The stiffer part of the cell body was fairly stable and did not show morphological changes for over 1 h. This is probably due to excess condensation of the actin network, hardening the cell cortex, and lowering the cytoskeletal activity. The nuclear portion of the cell body seems to be slightly less viscous than the peripheral region.