A likelihood ratio test for species membership based on DNA sequence data
A likelihood ratio test for species membership based on DNA sequence data
复制标题
DOI：
10.1098/rstb.2005.1728
复制
发表时间：
2005-10-29
影响因子：
6.3
通讯作者：
Nielsen, R
中科院分区：
文献类型：
--
作者：
Matz, MV;Nielsen, R
DNA条形码作为一种物种鉴定的方法正在迅速普及。然而，目前还不清楚哪些统计程序应该伴随着技术来提供不确定性的测量。在这里，我们描述了一个似然比测试，可用于测试，如果一个采样序列是一个先验指定的物种的成员。我们使用聚结模拟以及使用来自蝴蝶和青蛙的真实的数据研究了测试的性能，这些数据代表了DNA条形码的两种挑战：极低和极高水平的序列变异性。
DNA barcoding as an approach for species identification is rapidly increasing in popularity. However, it remains unclear which statistical procedures should accompany the technique to provide a measure of uncertainty. Here we describe a likelihood ratio test which can be used to test if a sampled sequence is a member of an a priori specified species. We investigate the performance of the test using coalescence simulations, as well as using the real data from butterflies and frogs representing two kinds of challenge for DNA barcoding: extremely low and extremely high levels of sequence variability.