Molecular cloning and analysis of the CRY1 gene: a yeast ribosomal protein gene.
Molecular cloning and analysis of the CRY1 gene: a yeast ribosomal protein gene.
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DOI：
10.1093/nar/11.2.403
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发表时间：
1983-01
影响因子：
14.9
通讯作者：
John C. Larkin;J. Woolford
中科院分区：
文献类型：
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作者：
John C. Larkin;J. Woolford
使用来自酵母交配型基因座（MAT）附近的克隆DNA作为探针，通过染色体步移技术已经分离出隐侧耳素抗性基因的野生型等位基因，并且已经显示与核糖体蛋白59的基因相同。还使用整合切除方法克隆了隐性cryR 1等位基因。MAT与BM 1的遗传距离为2.2 cM，而物理距离为21 kb，这一区间的比率约为10 kb/cM。该基因的两种质粒携带等位基因的表型表达可以在体内检测。使用这个基因作为杂交探针检查RNA加工缺陷的RNA 2，RNA 3，RNA 4，RNA 8和RNA 11突变体也进行了讨论。
Using cloned DNA from the vicinity of the yeast mating type locus (MAT) as a probe, the wild type allele of the cryptopleurine resistance gene CRY1 has been isolated by the technique of chromosome walking and has been shown to be identical to the gene for ribosomal protein 59. A recessive cryR1 allele has also been cloned, using the integration excision method. The genetic distance from MAT to CRY1 is 2.2 cM, while the physical distance is 21 kb, giving a ratio of about 10 kb/cM for this interval. The phenotypic expression of both plasmid borne alleles of the gene can be detected in vivo. The use of this gene as a hybridization probe to examine RNA processing defects in the rna 2, rna 3, rna 4, rna 8, and rna 11 mutants is also discussed.