Genomewide production of multipurpose alleles for the functional analysis of the mouse genome
Genomewide production of multipurpose alleles for the functional analysis of the mouse genome
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DOI：
10.1073/pnas.0502273102
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发表时间：
2005-05-17
影响因子：
11.1
通讯作者：
von Melchner, H
中科院分区：
文献类型：
--
作者：
Schnütgen, F;De-Zolt, S;von Melchner, H
一种逆转录病毒基因捕获载体已经被开发出来，它可以诱导小鼠胚胎干细胞（ES）中表达的大多数基因发生条件突变。这些载体依赖于定向位点特异性重组系统，当连续激活时，该系统可以修复和重新引入基因陷阱突变。基因陷阱插入小鼠基因组后，可以在体细胞中的特定时间和地点产生基因突变。除了它们的条件性特征之外，载体还产生适合于广泛的插入后修饰的多用途等位基因。在这里，我们已经使用这些定向重组载体组装了最大的ES细胞系文库，该文库具有尚未组装的单个基因中的条件突变，目前总计1，000个独特基因。被捕获的ES细胞系可以从德国基因捕获联盟订购，科学界可以免费获得。
A type of retroviral gene trap vectors has been developed that can induce conditional mutations in most genes expressed in mouse embryonic stem (ES) cells. The vectors rely on directional site-specific recombination systems that can repair and reinduce gene trap mutations when activated in succession. After the gene traps are inserted into the mouse genome, genetic mutations can be produced at a particular time and place in somatic cells. In addition to their conditional features, the vectors create multipurpose alleles amenable to a wide range of postinsertional modifications. Here we have used these directional recombination vectors to assemble the largest library of ES cell lines with conditional mutations in single genes yet assembled, presently totaling 1,000 unique genes. The trapped ES cell lines, which can be ordered from the German Gene Trap Consortium, are freely available to the scientific community.