Synthesis of the 5'-Se-thymidine phosphoramidite and convenient labeling of DNA oligonucleotide.
Synthesis of the 5'-Se-thymidine phosphoramidite and convenient labeling of DNA oligonucleotide.
复制标题
5-Se-胸苷亚磷酰胺的合成和 DNA 寡核苷酸的方便标记。
DOI：
10.1021/ol200397c
复制
发表时间：
2011
期刊：
影响因子：
5.2
通讯作者：
Huang,Zhen
中科院分区：
文献类型：
--
作者：
Zhang,Wen;Huang,Zhen
The synthesis of the 5′-Se-derivatized thymidine and phosphoramidite is reported, and the Se-phosphoramidite was incorporated into DNA. Because of its high nucleophilicity, this 5′-Se-moiety permits convenient postsynthetic modification of oligonucleotides. The proof of principle was successfully demonstrated by incorporating fluorescein into DNA. It was also found that the 5′-dye-labeled Se-DNA can be recognized by DNA polymerase as an efficient primer. This novel strategy opens a new avenue for nucleic acid probe preparation and detection.
