Controlled Release of Vascular Endothelial Growth Factor from Heparin-Functionalized Gelatin Type A and Albumin Hydrogels
Controlled Release of Vascular Endothelial Growth Factor from Heparin-Functionalized Gelatin Type A and Albumin Hydrogels
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DOI：
10.3390/gels3040035
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发表时间：
2017-12-01
期刊：
影响因子：
4.6
通讯作者：
Borchers, Kirsten
中科院分区：
文献类型：
--
作者：
Claassen, Christiane;Sewald, Lisa;Borchers, Kirsten
用于促血管生成生长因子的生物基释放系统是令人感兴趣的，以克服植入物的血管化和生物整合不足。在这项研究中，我们研究了肝素功能化的水凝胶的基础上明胶A型或白蛋白作为存储和释放系统的血管内皮生长因子（VEGF）。水凝胶在肝素存在下使用碳二亚胺化学进行交联。通过临界电解质浓度（CEC）染色监测水凝胶的肝素官能化。水凝胶的特征在于在缓冲溶液和含VEGF的溶液中的溶胀，并监测其负载和VEGF的释放。与明胶基凝胶相比，白蛋白基凝胶的平衡溶胀度（EDS）较低。对于两种生物聚合物，EDS可以根据所使用的碳二亚胺浓度进行调节。此外，VEGF负载和释放取决于碳二亚胺浓度和两种生物聚合物的负载条件。与明胶基凝胶相比，白蛋白基凝胶的负载更高，并且其突释更低。最后，与基于明胶A的水凝胶相比，确定了基于白蛋白的水凝胶在21天后升高的累积VEGF释放。我们认为蛋白质的特征性净电荷和释放期间白蛋白的降解是观察到的效应的原因。两种肝素功能化生物材料系统，化学交联明胶A型或白蛋白，具有可调的物理化学性质，并可被认为是控制输送促血管生成生长因子VEGF。
Bio-based release systems for pro-angiogenic growth factors are of interest, to overcome insufficient vascularization and bio-integration of implants. In this study, we investigated heparin-functionalized hydrogels based on gelatin type A or albumin as storage and release systems for vascular endothelial growth factor (VEGF). The hydrogels were crosslinked using carbodiimide chemistry in presence of heparin. Heparin-functionalization of the hydrogels was monitored by critical electrolyte concentration (CEC) staining. The hydrogels were characterized in terms of swelling in buffer solution and VEGF-containing solutions, and their loading with and release of VEGF was monitored. The equilibrium degree of swelling (EDS) was lower for albumin-based gels compared to gelatin-based gels. EDS was adjustable with the used carbodiimide concentration for both biopolymers. Furthermore, VEGF-loading and release were dependent on the carbodiimide concentration and loading conditions for both biopolymers. Loading of albumin-based gels was higher compared to gelatin-based gels, and its burst release was lower. Finally, elevated cumulative VEGF release after 21 days was determined for albumin-based hydrogels compared to gelatin A-based hydrogels. We consider the characteristic net charges of the proteins and degradation of albumin during release time as reasons for the observed effects. Both heparin-functionalized biomaterial systems, chemically crosslinked gelatin type A or albumin, had tunable physicochemical properties, and can be considered for controlled delivery of the pro-angiogenic growth factor VEGF.