Vitronectin inhibits the thrombotic response to arterial injury in mice
Vitronectin inhibits the thrombotic response to arterial injury in mice
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DOI：
10.1182/blood.v93.6.1825.406k37_1825_1830
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发表时间：
1999-03-15
期刊：
影响因子：
20.3
通讯作者：
Ginsburg, D
中科院分区：
文献类型：
--
作者：
Fay, WP;Parker, AC;Ginsburg, D
玻璃连结蛋白(vitronectin，VN)与纤溶酶原激活物抑制物-1(PAI-1)和整合素结合，可能通过调节纤溶和细胞迁移在血管损伤反应中发挥重要作用。然而，VN在血管损伤-血栓形成的最早反应中的作用还没有得到很好的描述。这项研究的目的是验证这一假说，即玻璃连结蛋白表达的变化改变了小鼠对动脉损伤的血栓反应。用三氯化铁(FeCl3)诱导小鼠颈动脉富血小板血栓形成。研究对象为年龄和性别匹配的野生型(VN+/+，n=14)和VN-/-(VN-/-，n=15)小鼠。FeCl3损伤后闭塞的时间通过对颈动脉应用多普勒血流探头来确定。VN-/-小鼠闭塞时间明显短于VN+/+小鼠(分别为6.0+/-1.2min和17.8+/-2.3min，P
Vitronectin (VN) binds to plasminogen activator inhibitor-1 (PAI-1) and integrins and may play an important role in the vascular response to injury by regulating fibrinolysis and cell migration. However, the role of VN in the earliest response to vascular injury, thrombosis, is not well characterized. The purpose of this study was to test the hypothesis that variation in vitronectin expression alters the thrombotic response to arterial injury in mice. Ferric chloride (FeCl3) injury was used to induce platelet-rich thrombi in mouse carotid arteries. Wild-type (VN +/+, n = 14) and VN-deficient (VN -/-, n = 15) mice, matched for age and gender, were studied. Time to occlusion after FeCl3 injury was determined by application of a Doppler flowprobe to the carotid artery. Occlusion times of VN -/- mice were significantly shorter than those of VN +/+ mice (6.0 +/- 1.2 minutes v 17.8 +/- 2.3 minutes, respectively, P