Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway
Melatonin benefits to the growth of human annulus fibrosus cells through inhibiting miR-106a-5p/ATG7 signaling pathway
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DOI：
10.2147/cia.s193765
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发表时间：
2019-01-01
影响因子：
3.6
通讯作者：
Liu, Xiaoguang
中科院分区：
文献类型：
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作者：
Hai, Bao;Ma, Yunlong;Liu, Xiaoguang
背景：椎间盘退变（disc degeneration，DD）是世界范围内的常见病之一，严重影响人们的正常生活，给人们带来难以忍受的疼痛。方法：本研究系统观察了褪黑素对DD患者纤维环（annulus fibrosus，AF）细胞的影响。结果：褪黑素对DD患者AF细胞具有促进增殖、诱导自噬、抑制凋亡的作用。此外，褪黑素可促进AF细胞自噬相关蛋白ATG 7的翻译和转录，抑制miR-106 a-5 p的功能。此外，结果表明miR-106 a-5 p通过直接结合AF细胞中ATG 7的3 'UTR来介导ATG 7的表达。结论：该研究不仅深入了解了褪黑激素的作用方式，而且还表明了其在AF细胞中潜在的靶信号通路。
Background: Disc degeneration (DD) is one of the common diseases worldwide, which deeply influences normal life and leads to excruciating pain. However, an effective treatment for DD is still not identified.Method: The present study systemically examined the effect of melatonin on annulus fibrosus (AF) cells of patients with DD.Results: Melatonin had the effect of promoting proliferation, inducing autophagy, and suppressing apoptosis on AF cells of patients with DD. Moreover, melatonin contributed to the translation and transcription of autophagy-related protein ATG7 and inhibited the function of miR-106a-5p in AF cells. In addition, the results suggested that miR-106a-5p mediated the expression of ATG7 by directly binding to its 3'UTR in AF cells.Conclusion: This research not only gained a deep insight of melatonin mode of action, but also indicated its potential target signaling pathway in AF cells.