Overexpression of CsrA (BB0184) alters the morphology and antigen profiles of Borrelia burgdorferi.
Overexpression of CsrA (BB0184) alters the morphology and antigen profiles of Borrelia burgdorferi.
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CsrA (BB0184) 的过度表达会改变伯氏疏螺旋体的形态和抗原谱。
DOI：
10.1128/iai.00673-09
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发表时间：
2009
影响因子：
3.1
通讯作者：
Seshu,J
中科院分区：
文献类型：
--
作者：
Sanjuan,Eva;Esteve-Gassent,MariaD;Maruskova,Mahulena;Seshu,J
莱姆病的病原体伯氏疏螺旋体改变其基因表达，以响应其宿主中遇到的高度不同的环境信号。疏螺旋体基因组中存在的适应性基因表达的相对较少的调节因子中有一个开放阅读框（ORF），BB 0184，注释为CsrA（carbonstoragegregulatorA）。在几种细菌中，CsrA已被表征为小RNA结合蛋白，其作为影响多个ORF的mRNA稳定性或翻译水平的全局调节剂起作用。与已知的CsrA功能一致，从B.在大肠杆菌中，伯氏螺旋体（CsrABb）导致糖原积累减少。我们确定，CsrAB是FlgK运动操纵子的一部分，当B.伯氏菌在食蜱条件下繁殖。CsrABbinB的过表达。Burgdorferisstrain B31（ML 23，lp 25-阴性克隆分离物）产生命名为ES 25的克隆，其表现出菌落形态的改变和FlaB水平的显著降低。先前表征为在感染性中起作用的几种脂蛋白也在ES 25中改变。RNA的实时逆转录-PCR分析显示ES 25中ofospC的转录水平存在显着差异，而其他转录物的水平则没有这种差异，这表明后者基因的表达存在转录后调节。这些观察结果表明CsrAB在B.对CsrAB b的进一步鉴定将有助于更好地了解B. burgdorferi。
Borrelia burgdorferi, the agent of Lyme disease, alters its gene expression in response to highly disparate environmental signals encountered in its hosts. Among the relatively few regulators of adaptive gene expression present in the borrelial genome is an open reading frame (ORF), BB0184, annotated as CsrA (carbonstorageregulatorA). CsrA, in several bacterial species, has been characterized as a small RNA binding protein that functions as a global regulator affecting mRNA stability or levels of translation of multiple ORFs. Consistent with known functions of CsrA, overexpression of CsrA fromB. burgdorferi(CsrABb) inEscherichia coliresulted in reduced accumulation of glycogen. We determined thatcsrABbis part of theflgKmotility operon and that the synthesis of CsrABbwas increased whenB. burgdorferiwas propagated under fed-tick conditions. Overexpression of CsrABbinB. burgdorferistrain B31 (ML23, lp25-negative clonal isolate) resulted in a clone, designated ES25, which exhibited alterations in colony morphology and a significant reduction in the levels of FlaB. Several lipoproteins previously characterized as playing a role in infectivity were also altered in ES25. Real-time reverse transcription-PCR analysis of RNA revealed significant differences in the transcriptional levels ofospCin ES25, while there were no such differences in the levels of other transcripts, suggesting posttranscriptional regulation of expression of these latter genes. These observations indicate that CsrABbplays a role in the regulation of expression of pathophysiological determinants ofB. burgdorferi, and further characterization of CsrABbwill help in better understanding of the regulators of gene expression inB. burgdorferi.