Seminal level of clusterin in infertile men as a significant biomarker reflecting spermatogenesis
Seminal level of clusterin in infertile men as a significant biomarker reflecting spermatogenesis
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DOI：
10.1111/and.12558
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发表时间：
2016-12-01
期刊：
影响因子：
2.4
通讯作者：
Fujisawa, M.
中科院分区：
文献类型：
--
作者：
Fukuda, T.;Miyake, H.;Fujisawa, M.
本研究的目的是评估精子聚集素水平对不育男性精子发生的影响。本研究包括89名因不孕症就诊的男性，其中正常精子症28例，少精子症33例，非梗阻性无精子症（NOA）28例。用酶联免疫吸附法测定正常、少精子症和NOA患者精浆聚集素浓度分别为47.9、28.2和18.4ng/ml，三组间差异有显著性（P < 0.01）。对28例NOA患者行睾丸显微切割精子提取术（MD-TESE），9例成功回收精子。在NOA患者中，精子聚集素水平与睾丸聚集素蛋白表达之间存在显著相关性（P = 0.026）。在MD-TESE前可用的几个参数中，单变量分析确定血清促卵泡激素（FSH）水平< 10 IU ml（-1）和精液凝集素水平>= 18 ng ml（-1）是精子回收的重要预测因子，其中，只有血清FSH水平< 10 IU ml（-1）在多变量分析中显示与精子回收独立相关。因此，精浆凝集素水平作为一种生物标志物在不育男性生精状态评估中的意义值得进一步探讨。
The objective of this study was to assess the impact of seminal clusterin level on spermatogenesis in infertile men. This study included 89 men who visited our clinic due to infertility, consisting of 28, 33, and 28 diagnosed with normospermia, oligozoospermia and nonobstructive azoospermia (NOA) respectively. The seminal clusterin concentrations measured by enzyme-linked immunosorbent assay were 47.9, 28.2 and 18.4 ng ml(-1) in men with normospermia, oligozoospermia and NOA, respectively, with significant differences among these three groups (P < 0.01). Microdissection testicular sperm extraction (MD-TESE) was performed in the 28 men with NOA, and spermatozoon was successfully retrieved from 9. There was a significant correlation between seminal clusterin level and testicular clusterin protein expression evaluated by immunohistochemical staining in these men with NOA (P = 0.026). Of several parameters available before MD-TESE, the univariate analysis identified serum follicle-stimulating hormone (FSH) level < 10 IU ml(-1) and seminal clusterin level >= 18 ng ml(-1) as significant predictors of sperm retrieval, and of these, only serum FSH level < 10 IU ml(-1) was shown to be independently associated with sperm retrieval in the multivariate analysis. Accordingly, it might be worthy to further evaluate the significance of seminal clusterin level as a biomarker for the assessment of spermatogenic status in infertile men.