Effects of stress on alveolar macrophages: A role for the sympathetic nervous system
Effects of stress on alveolar macrophages: A role for the sympathetic nervous system
复制标题
DOI：
10.1165/ajrcmb.19.5.3103
复制
发表时间：
1998-11-01
影响因子：
6.4
通讯作者：
Kraal, G
中科院分区：
文献类型：
--
作者：
Broug-Holub, E;Persoons, JHA;Kraal, G
肺泡巨噬细胞（Alveolar macrophages，AM）在肺局部免疫反应的调节中起重要作用。先前表明，大鼠暴露于轻度不可避免的电足电击应激（20分钟，4次电击/分钟，5秒/电击，0.8毫秒）导致刺激后AM活性的明显变化，与从非应激大鼠分离的AM的分泌相比，反映为增强的白细胞介素-1 β和肿瘤坏死因子-α分泌和减少的一氧化氮分泌。在这里，我们表明，在体内阻断自主神经系统的烟碱受体拮抗剂氯异山达明腹腔注射导致这些应力诱导的AM活性的改变完全废除。自主神经系统的这种作用可进一步归因于β-肾上腺素能受体的交感神经刺激，如β-肾上腺素能受体的阻断所示。无论是α-肾上腺素受体或副交感神经输出的封锁并没有导致废除的应激诱导的AM活性的变化。AM活性的β-肾上腺素能调节最有可能不是由于AM上的儿茶酚胺的直接作用，因为模拟在体内的应激作用，通过在体外预孵育AM与不同剂量的儿茶酚胺，然后通过脂多糖刺激，并没有导致改变AM的细胞因子分泌。
Alveolar macrophages (AMs) play an important role in the regulation of the local immune reactivity in the lung. It was previously shown that exposure of rats to mild inescapable electrical footshock stress (20 min, 4 shocks/min, 5 s/shock, 0.8 mAmp) leads to apparent changes in the activity of AMs upon stimulation, reflected by an enhanced interleukin-1 beta and tumor necrosis factor-alpha secretion and decreased nitric oxide secretion compared with the secretion by AMs isolated from nonstressed rats. Here we show that in vivo blockade of the autonomic nervous system by intraperitoneal injection of the nicotinic receptor antagonist chlorisondamine leads to complete abrogation of these stress-induced alterations in AM activity. This role fur the autonomic nervous system could further be attributed to sympathetic stimulation of beta-adrenergic receptors as shown by blockade of beta-adrenoceptors. Blockade of either alpha-adrenoceptors or parasympathetic output did not result in abrogation of the stress-induced changes in AM activity. The beta-adrenergic modulation of AM activity most likely is not due to a direct effect of catecholamines on AMs because mimicking the in vivo stress effects by in vitro preincubation of AMs with various doses of catecholamines followed by lipopolysaccharide stimulation did not result in an altered cytokine secretion by AMs.