GENERATION OF SPONTANEOUS RESPIRATORY RHYTHM IN HIGH SPINAL CATS
GENERATION OF SPONTANEOUS RESPIRATORY RHYTHM IN HIGH SPINAL CATS
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DOI：
10.1016/s0006-8993(80)80034-5
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发表时间：
1980-01-01
期刊：
影响因子：
2.9
通讯作者：
EBATA, N
中科院分区：
文献类型：
--
作者：
AOKI, M;MORI, S;EBATA, N
在36只成年猫（24只颈脊髓和12只非颈脊髓标本）上观察了颈脊髓内自发呼吸神经元的活动。在18只动物中，C1全脊髓横断后自主呼吸消失。13只动物在横断后2小时内再次出现自主节律性呼吸。在6只C3节段脊髓损伤的动物中，不能诱导自主呼吸。脊髓呼吸运动主要是由于有节奏的横隔膜收缩造成的。脊髓呼吸活动持续30-60 min，频率稳定在19-24次/min，然后逐渐减弱。脊髓呼吸活动通常发展为后肢肌肉活动，即使同时观察到后肢步进节律，也不锁定于呼吸节律。在脊髓呼吸过程中，膈运动神经元放电记录从C5-C6腹角。爆发性放电包含4.8-40.0个棘波，与呼吸周期的吸气相同步。即使在呼吸运动被加拉明暂时麻痹后，有节奏的爆发仍然持续了相当长的时间。在12只轻度麻醉的猫中，对上颈髓进行了微电极探索，试图记录与呼吸运动相关的神经元活动。记录了代表假定细胞体活动的吸气型单位的爆发放电（24）。记录部位在组织学上位于C1-C2颈髓脊髓灰质中间带。固有呼吸节律发生器可能存在于颈髓内。自发呼吸节律的产生可能的神经机制进行了讨论。
Spontaneous respiratory neuronal activities within cervical spinal cord were investigated in 36 adult cats: 24 cervical spinalized and 12 non-spinalized preparations. In 18 animals, spontaneous breathing was abolished after total spinal transection at C1. Spontaneous rhythmic breathing reappeared within 2 h after transection in 13 animals. In 6 animals spinalized at C3 level, spontaneous breathing could not be induced. The spinal respiratory movements were mainly due to rhythmic diaphragmatic contraction. Such spinal respiratory activity continused for 30-60 min, with a steady rate of 19-24/min and then steadily deteriorated. Spinal respiratory activity developed usually hindlimb muscle activity and even when hindlimb stepping rhythm was seen simultaneously, it was not locked to respiratory rhythmicity. During spinal respiration, phrenic motoneuron discharges were recorded from the C5-C6 ventral horn. The burst discharges containing 4.8-40.0 spikes were in synchrony with the inspiratory phase of respiratory cycles. Even after breathing movements were temporarily paralyzed by gallamine, the rhythmic bursts still persisted for an appreciable time. In 12 lightly anesthetized cats, microelectrode explorations of the upper cervical cord were made in an attempt to record neuronal activities associated with respiratory movements. Burst discharges (24) of inspiratory type units which represent presumed cell body activities were recorded. The recording sites were histologically located in the intermediate zone of the spinal gray matter of C1-C2 cervical cord. Intrinsic respiratory rhythm generators may exist within the cervical cord. Possible neuronal mechanisms for generation of spontaneous respiratory rhythm are discussed.