CORRELATIONS BETWEEN THE C-13 CONTENT OF PRIMARY AND SECONDARY PLANT-PRODUCTS IN DIFFERENT CELL COMPARTMENTS AND THAT IN DECOMPOSING BASIDIOMYCETES
CORRELATIONS BETWEEN THE C-13 CONTENT OF PRIMARY AND SECONDARY PLANT-PRODUCTS IN DIFFERENT CELL COMPARTMENTS AND THAT IN DECOMPOSING BASIDIOMYCETES
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DOI：
10.1104/pp.102.4.1287
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发表时间：
1993-08-01
期刊：
影响因子：
7.4
通讯作者：
SCHMIDT, HL
中科院分区：
文献类型：
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作者：
GLEIXNER, G;DANIER, HJ;SCHMIDT, HL
已测定了一年生植物、松针、木材和分解担子菌不同区室的初级和次级产物的相对碳同位素比（deltaC-13 值）。由于主要贮藏产物蔗糖和淀粉含量较高，一年生植物的贮藏组织中 C-13 含量较高；然而，叶淀粉的富集程度更高。所有这些化合物在葡萄糖的 3 和 4 位上具有相同的相对 C-13 富集度。针叶树针中的次级产品（木质素、脂质）中的 C-13 相对于同一来源的碳水化合物而言减少了千分之 1 至 2。空气污染导致 deltaC-13 值小幅下降；然而，植物产品的相对含量，特别是可溶性极性化合物的相对含量也受到影响。相对于其在木材中的基质，分解真菌显示出 C-13 的全球累积量为千分之 4。相对于木材的纤维素，它们的甲壳素含量增加了千分之二。因此，担子菌优先代谢“轻”分子，而“重”分子优先聚合。我们的结果是基于同位素对果糖-1,6-二磷酸醛缩酶反应的动力学效应以及不同底物通量的磷酸丙糖水平上的代谢分支的影响进行讨论的。
Relative carbon isotope ratio (deltaC-13 values) of primary and secondary products from different compartments of annual plants, pine needles, wood, and decomposing Basidiomycetes have been determined. An enrichment in C-13 was found for storage tissues of annual plants, because of the high level of the primary storage products sucrose and starch; however, the enrichment was even greater in leaf starch. All of these compounds had the same relative C-13 enrichment in positions 3 and 4 of glucose. Secondary products in conifer needles (lignin, lipids) were depleted in C-13 by 1 to 2 parts per thousand relative to carbohydrates from the same origin. Air pollution caused a small decrease in deltaC-13 values; however, the relative content of plant products, especially of the soluble polar compounds, was also affected. Decomposing fungi showed a global accumulation of C-13 by 4 parts per thousand relative to their substrates in wood. Their chitin was enriched by 2 parts per thousand relative to the cellulose of the wood. Hence, Basidiomycetes preferentially metabolize ''light'' molecules, whereas ''heavy'' molecules are preferentially polymerized. Our results are discussed on the basis of a kinetic isotope effect on the fructose-1,6-bisphosphate aldolase reaction and of metabolic branching on the level of the triose phosphates with varying substrate fluxes.