Chaperone complex formation of the transcription factor MalR involved in maltose utilization and amylolytic enzyme production in Aspergillus oryzae
Chaperone complex formation of the transcription factor MalR involved in maltose utilization and amylolytic enzyme production in Aspergillus oryzae
复制标题
米曲霉中参与麦芽糖利用和淀粉分解酶产生的转录因子 MalR 的伴侣复合物形成
DOI：
10.1080/09168451.2018.1447359
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
K. Gomi
中科院分区：
文献类型：
--
作者：
Y. Konno;K. Suzuki;M. Tanaka;T. Shintani;K. Gomi
Zn2Cys6 型转录因子 MalR 控制米曲霉中麦芽糖利用 (MAL) 簇基因的表达和淀粉分解酶的产生。在本研究中，我们证明 MalR 在类似于酵母对应物 Mal63 的非诱导条件下与 Hsp70 和 Hsp90 伴侣形成复合物，并且在添加诱导剂麦芽糖后，该复合物从伴侣复合物中释放出来。 MalR 蛋白组成性定位于细胞核中，位于锌指基序和 C 末端区域的假定核定位信号 (NLS) 的突变导致核定位丧失。这一结果表明 NSL 参与 MalR 核定位。然而，两个 NLS 中的突变并不影响 MalR-Hsp70/Hsp90 复合物的解离模式，这表明麦芽糖诱导的 MalR 激活可能会发生，无论其细胞内定位如何。
The Zn2Cys6-type transcription factor MalR controls the expression of maltose-utilizing (MAL) cluster genes and the production of amylolytic enzymes inAspergillus oryzae. In the present study, we demonstrated that MalR formed a complex with Hsp70 and Hsp90 chaperones under non-inducing conditions similar to the yeast counterpart Mal63 and that the complex was released from the chaperone complex after the addition of the inducer maltose. The MalR protein was constitutively localized in the nucleus and mutation in both the putative nuclear localization signals (NLSs) located in the zinc finger motif and the C-terminal region resulted in the loss of nuclear localization. This result indicated the involvement of NSLs in the MalR nuclear localization. However, mutation in both NLSs did not affect the dissociation mode of the MalR-Hsp70/Hsp90 complex, suggesting that MalR activation induced by maltose can occur regardless of its intracellular localization.