Citrus flavonoids repress the mRNA for stearoyl-CoA desaturase, a key enzyme in lipid synthesis and obesity control, in rat primary hepatocytes.
Citrus flavonoids repress the mRNA for stearoyl-CoA desaturase, a key enzyme in lipid synthesis and obesity control, in rat primary hepatocytes.
复制标题
DOI：
10.1186/1476-511x-10-36
复制
发表时间：
2011-02-23
影响因子：
4.5
通讯作者：
Holland LJ
中科院分区：
文献类型：
--
作者：
Nichols LA;Jackson DE;Manthey JA;Shukla SD;Holland LJ
柑橘类黄酮已被证明可以降低血脂水平，改善葡萄糖耐量，减轻肥胖。这些生理效应的一个可能机制是肝脏中硬脂酰辅酶a去饱和酶-1 (SCD1) mRNA水平的降低，因为这种酶的抑制可以减少高脂血症和肥胖。在这里，我们发现两种结构类的柑橘类黄酮以剂量依赖的方式降低大鼠原代肝细胞中SCD1 mRNA的浓度。这是柑橘类黄酮首次在体内或培养细胞中抑制SCD1。此外，这是首次使用从任何动物新鲜分离的肝细胞来检测柑橘类黄酮在mRNA水平上的作用。本研究表明，柑橘类黄酮对SCD1基因表达的调控可能参与了柑橘类黄酮对肥胖的控制，大鼠原代肝细胞是分析柑橘类黄酮在肝脏中作用的分子机制的生理相关模型系统。
Citrus flavonoids have been shown to decrease plasma lipid levels, improve glucose tolerance, and attenuate obesity. One possible mechanism underlying these physiological effects is reduction of hepatic levels of the mRNA for stearoyl-CoA desaturase-1 (SCD1), since repression of this enzyme reduces hyperlipidemia and adiposity. Here, we show that citrus flavonoids of two structural classes reduce SCD1 mRNA concentrations in a dose-dependent manner in rat primary hepatocytes. This is the first demonstration of repression of SCD1 by citrus flavonoids, either in vivo or in cultured cells. Furthermore, it is the first use of freshly-isolated hepatocytes from any animal to examine citrus flavonoid action at the mRNA level. This study demonstrates that regulation of SCD1 gene expression may play a role in control of obesity by citrus flavonoids and that rat primary hepatocytes are a physiologically-relevant model system for analyzing the molecular mechanisms of flavonoid action in the liver.