The Effects of Astragalus Polysaccharide on Bone Marrow-Derived Mesenchymal Stem Cell Proliferation and Morphology Induced by A549 Lung Cancer Cells
The Effects of Astragalus Polysaccharide on Bone Marrow-Derived Mesenchymal Stem Cell Proliferation and Morphology Induced by A549 Lung Cancer Cells
复制标题
黄芪多糖对A549肺癌细胞诱导的骨髓间充质干细胞增殖及形态的影响
DOI：
10.12659/msm.914219
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发表时间：
2019-06-02
影响因子：
3.1
通讯作者：
Guan, Quanlin
中科院分区：
文献类型：
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作者：
Zhang, Yue-Mei;Liu, Yong-Qi;Guan, Quanlin
背景：肺癌的肿瘤微环境在肿瘤的进展和转移中起着重要作用。骨髓间充质干细胞（MSCs）与A549肺癌细胞共培养后，细胞形态发生改变，细胞增殖增加，细胞迁移能力增强。本研究旨在探讨中药黄芪多糖（Astragalus多糖，APS）对体外培养A549肺癌细胞诱导的骨髓源性间质干细胞变化的影响。材料/方法：骨髓源性间充质干细胞与A549细胞共培养（Co-BMSCs）。用50 μ g/ml APS （Co-BMSCs + APS）处理共培养的骨髓源性MSCs和A549细胞与未处理的Co-BMSCs进行比较。采用细胞计数试剂盒-8 （CCK-8）法测定细胞增殖。流式细胞术评估细胞周期。采用微阵列法检测mRNA表达，Western blot法检测蛋白表达。结果：与未处理的Co-BMSCs相比，APS处理可改善Co-BMSCs的细胞形态，降低细胞增殖，抑制细胞周期阻滞。丝裂原活化蛋白激酶(MAPK)/核因子κ B （nf - κ B）途径、TP53、caspase-3、乙酰化H4K5、乙酰化H4K8和乙酰化H3K9参与了调控过程。结论：APS处理可减少体外培养的A549肺癌细胞与骨髓源性MSCs的细胞增殖和形态学改变。
Background: The tumor microenvironment in lung cancer plays an important role in tumor progression and metastasis. Bone marrow-derived mesenchymal stem cells (MSCs) co-cultured with A549 lung cancer cells show changes in morphology, increase cell proliferation, and cell migration. This study aimed to investigate the effects of Astragalus polysaccharide (APS), a traditional Chinese herbal medicine, on the changes induced in bone marrow-derived MSCs by A549 lung cancer cells in vitro.Material/Methods: Bone marrow-derived MSCs were co-cultured with A549 cells (Co-BMSCs). Co-cultured bone marrow-derived MSCs and A549 cells treated with 50 mu g/ml of APS (Co-BMSCs + APS) were compared with untreated Co-BMSCs. Cell proliferation was measured using the cell counting kit-8 (CCK-8) assay. Flow cytometry evaluated the cell cycle. Microarray assays for mRNA expression and Western blot for protein expression were used.Results: Compared with untreated Co-BMSCs, APS treatment of Co-BMSCs improved cell morphology, reduced cell proliferation, and inhibited cell cycle arrest. The mitogen-activated protein kinase (MAPK)/nuclear factor-kappa B (NF-kappa B) pathway, TP53, caspase-3, acetylated H4K5, acetylated H4K8, and acetylated H3K9 were involved in the regulatory process.Conclusions: APS treatment reduced cell proliferation and morphological changes in bone marrow-derived MSCs that were co-cultured with A549 lung cancer cells in vitro.