THE TOLL GENE OF DROSOPHILA, REQUIRED FOR DORSAL-VENTRAL EMBRYONIC POLARITY, APPEARS TO ENCODE A TRANSMEMBRANE PROTEIN
THE TOLL GENE OF DROSOPHILA, REQUIRED FOR DORSAL-VENTRAL EMBRYONIC POLARITY, APPEARS TO ENCODE A TRANSMEMBRANE PROTEIN
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DOI：
10.1016/0092-8674(88)90516-8
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发表时间：
1988-01-29
期刊：
影响因子：
64.5
通讯作者：
ANDERSON, KV
中科院分区：
文献类型：
--
作者：
HASHIMOTO, C;HUDSON, KL;ANDERSON, KV
果蝇的Toll基因是一种母性效应基因，在胚胎背腹模式的建立中起着核心作用。将克隆的DNA与选择素杂交，纯化得到5.3kb的PolyA+卵巢转录本。这一纯化的转录本补充了Toll突变的表型，这证明它是Toll转录本。序列分析表明，Toll蛋白是一种完整的膜蛋白，具有胞内区和较大的胞外区。推测的胞外结构域包含至少15个重复的24个氨基酸的富含亮氨酸的序列，在人和酵母膜蛋白中都存在。
The Toll gene of Drosophila, a maternal effect gene that plays a central role in the establishment of the embryonic dorsal-ventral pattern, has been cloned using P element tagging. A 5.3 kb poly(A)+ ovarian transcript from the cloned region was purified by hybrid selectin with the cloned DNA. This purified transcript complements the Toll mutant phenotype when injected into Toll- embryos, which proves that it is the Toll transcript. The sequence of cDNAs suggests that the Toll protein is an integral membrane protein with a cytoplasmic domain and a large extracytoplasmic domain. The putative extracytoplasmic domain contains at least 15 repeats of a 24 amino acid, leucine-rich sequence found in both human and yeast membrane proteins.