Synthesis and properties of geometrical 4-diarylmethylene analogs of the green fluorescent protein chromophore
Synthesis and properties of geometrical 4-diarylmethylene analogs of the green fluorescent protein chromophore
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DOI：
10.1039/c8ob00208h
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发表时间：
2018-04-14
影响因子：
3.2
通讯作者：
Miyashita, Kazuyuki
中科院分区：
文献类型：
--
作者：
Ikejiri, Masahiro;Kojima, Haruka;Miyashita, Kazuyuki
我们已经开发了一种新的类似物的GFP发色团：地理DAIN。由于geo-DAIN配备有E/Z-可光异构化的几何二芳基亚甲基部分而不是GFP发色团的亚苄基，因此预期不同颜色的可逆发射。我们通过与亚胺酸甲酯和N-（二芳基亚甲基）甘氨酸的缩合反应合成了geo-DAIN。我们发现geo-DAIN的发射与亚苄基型类似物的发射不同;在粉末状态下，geo-DAIN的E-和Z-异构体发射不同的荧光颜色。
We have developed a novel analog of the GFP chromophore: geo-DAIN. Since geo-DAIN is equipped with an E/Z-photoisomerizable geometrical diarylmethylene moiety instead of benzylidene of the GFP chromophore, different-colored reversible emissions are expected. We synthesized geo-DAIN by a condensation with methyl imidate and N-(diarylmethylene)glycinate. We found the emission from geo-DAIN to be different from that of benzylidene-type analogs; in the powder state, the E- and Z-isomers of geo-DAIN emitted different fluorescence colors.