A role for creD, a carbon catabolite repression gene from Aspergillus nidulans, in ubiquitination
A role for creD, a carbon catabolite repression gene from Aspergillus nidulans, in ubiquitination
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DOI：
10.1111/j.1365-2958.2004.04172.x
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发表时间：
2004-08-01
影响因子：
3.6
通讯作者：
Kelly, JM
中科院分区：
文献类型：
--
作者：
Boase, NA;Kelly, JM
在构巢曲霉中，已知 creB 编码一种去泛素化酶，该酶与包含 creC 编码的蛋白质的 WD40 基序形成复合物，这些基因的突变导致碳源利用改变，而 creD34 突变抑制 creC 和 creB 突变的表型效应。因此，对 creD 进行表征是为了剖析涉及 CreB-CreC 去泛素化复合物的调控网络。 CreD 包含抑制蛋白结构域和 PY 基序，与酿酒酵母的 Rod1p 和 Rog3p 蛋白高度相似。在构巢曲霉基因组中鉴定出另一个基因，该基因也编码含有视紫红质抑制蛋白和 PY 基序的蛋白质，我们将其命名为 apyA，因此构巢曲霉中也存在两种类似的蛋白质。在酿酒酵母中，Rod1p和Rog3p与泛素连接酶Rsp5p相互作用，因此鉴定了Rsp5p的构巢曲霉同源物，编码该HECT泛素连接酶的基因被命名为hulA。通过细菌双杂交系统测试了CreD和ApyA与HulA的蛋白质-蛋白质相互作用，在该系统中ApyA与HulA表现出强相互作用，而CreD与HulA表现出弱相互作用。
In Aspergillus nidulans, it is known that creB encodes a deubiquitinating enzyme that forms a complex with the WD40 motif containing protein encoded by creC, that mutations in these genes lead to altered carbon source utilization and that the creD34 mutation suppresses the phenotypic effects of mutations in creC and creB. Therefore, creD was characterized in order to dissect the regulatory network that involves the CreB-CreC deubiquitination complex. CreD contains arrestin domains and PY motifs and is highly similar to the Rod1p and Rog3p proteins from Saccharomyces cerevisiae. An additional gene was identified in the A. nidulans genome that also encodes an arrestin and PY motif-containing protein, which we have designated apyA, and thus two similar proteins also exist in A. nidulans. In S. cerevisiae, Rod1p and Rog3p interact with the ubiquitin ligase Rsp5p, and so the A. nidulans homologue of Rsp5p was identified, and the gene encoding this HECT ubiquitin ligase was designated hulA. CreD and ApyA were tested for protein-protein interactions with HulA via the bacterial two-hybrid system, and ApyA showed strong interaction, and CreD showed weak interaction, with HulA in this system.