Asymmetrical Deterministic Lateral Displacement Gaps for Dual Functions of Enhanced Separation and Throughput of Red Blood Cells.
Asymmetrical Deterministic Lateral Displacement Gaps for Dual Functions of Enhanced Separation and Throughput of Red Blood Cells.
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DOI：
10.1038/srep22934
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发表时间：
2016-03-10
影响因子：
4.6
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Zeming KK;Salafi T;Chen CH;Zhang Y
确定性横向位移（DLD）微流控装置颗粒分离方法以其高分辨率和鲁棒性得到了广泛的应用。DLD已经显示出广泛应用的多功能性，用于寄生虫、血细胞、细菌和DNA等微颗粒的分选。DLD模型是为球形颗粒而设计的，由于血细胞的形状和大小不均匀，对有效分离血细胞具有挑战性。此外，在亚微米范围内的分离需要减小DLD系统的间隙尺寸，这将成倍地增加器件电阻，从而大大降低吞吐量。本文展示了如何通过改变横向间隙（GL）与下游间隙（GD）的比例来简单地应用不对称DLD间隙大小，从而在不大大限制吞吐量的情况下实现有效的红细胞分离。该方法减少了DLD柱的挑战性制造，并为当前的DLD模型提供了新的见解。分离显示DLD临界直径分辨率（分离较小的颗粒）的增加和非球形红细胞的选择性增加。与具有对称间隙大小的标准DLD模型相比，红细胞分离更好。该方法可用于分离非球形细菌或亚微米颗粒，以提高通量和DLD分辨率。
Deterministic lateral displacement (DLD) method for particle separation in microfluidic devices has been extensively used for particle separation in recent years due to its high resolution and robust separation. DLD has shown versatility for a wide spectrum of applications for sorting of micro particles such as parasites, blood cells to bacteria and DNA. DLD model is designed for spherical particles and efficient separation of blood cells is challenging due to non-uniform shape and size. Moreover, separation in sub-micron regime requires the gap size of DLD systems to be reduced which exponentially increases the device resistance, resulting in greatly reduced throughput. This paper shows how simple application of asymmetrical DLD gap-size by changing the ratio of lateral-gap (GL) to downstream-gap (GD) enables efficient separation of RBCs without greatly restricting throughput. This method reduces the need for challenging fabrication of DLD pillars and provides new insight to the current DLD model. The separation shows an increase in DLD critical diameter resolution (separate smaller particles) and increase selectivity for non-spherical RBCs. The RBCs separate better as compared to standard DLD model with symmetrical gap sizes. This method can be applied to separate non-spherical bacteria or sub-micron particles to enhance throughput and DLD resolution.