Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans.
Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans.
复制标题
L-谷氨酰胺的鉴定：环状芽孢杆菌中布蒂罗辛生物合成所需的 2-脱氧青蟹肌糖转氨酶。
DOI：
10.7164/antibiotics.55.707
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
K. Kakinuma
中科院分区：
文献类型：
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作者：
H. Tamegai;E. Nango;Mieko Kuwahara;Hideki Yamamoto;Yasumasa Ota;Hisako Kuriki;T. Eguchi;K. Kakinuma
利用反向聚合酶链式反应，在环状芽孢杆菌丁松素生物合成BtR基因簇的糖基转移酶基因btrM的上游发现了两个新基因(btrN和btrs)。该上游基因与灰色链霉菌的STSC基因有显著的同源性，该基因编码链霉素生物合成中的L-谷氨酰胺：蔗糖转氨酶。BTRs在大肠杆菌中的异源表达和2-脱氧半胱氨酸肌糖转化为2-脱氧半胱氨酸肌胺的化学鉴定进一步证实了BTRs的功能。BTRs被鉴定为L-谷氨酰胺：2-脱氧半胱氨酸氨基转移酶，这是有关2-脱氧链胺生物合成的氨基转移酶基因的首次报道。
Using inverse PCR, two new genes (btrN and btrS) were identified upstream of the putative glycosyltransferase gene btrM in the butirosin-biosynthetic btr gene cluster of Bacillus circulans. The upstream gene btrS showed significant homology with stsC of Streptomyces griseus, which encodes L-glutamine:scyllo-inosose aminotransferase in the biosynthesis of streptomycin. The function of BtrS was further confirmed by heterologous expression in Escherichia coli and chemical identification of the conversion of 2-deoxy-scyllo-inosose into 2-deoxy-scyllo-inosamine. The identification of BtrS as L-glutamine:2-deoxy-scyllo-inosose aminotransferase is the first report of the aminotransferase gene responsible for 2-deoxystreptamine biosynthesis.