Human metallothionein genes: molecular cloning and sequence analysis of the mRNA.
Human metallothionein genes: molecular cloning and sequence analysis of the mRNA.
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人类金属硫蛋白基因：mRNA 的分子克隆和序列分析。
DOI：
10.1093/nar/10.10.3165
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发表时间：
1982
影响因子：
14.9
通讯作者：
Robert I. Richards
中科院分区：
文献类型：
--
作者：
Michael Karin;Robert I. Richards
从镉处理的HeLa细胞制备的cDNA克隆库中，我们分离到了代表镉诱导后浓度升高的mRNAs的克隆。通过与小鼠金属硫蛋白-I基因的杂交，获得了多个金属硫蛋白基因的克隆。对其中一个克隆的核苷酸序列进行了测定，该克隆包含一个几乎全长的人金属硫蛋白-II m RNA的c DNA拷贝。人和小鼠金属硫蛋白序列之间的同源性严格限制在mRNA的编码区。金属硫蛋白基因密码子的使用不是随机的。79%的密码子在第三位有G或C残基，导致富含GC的序列。
From a cDNA clone bank prepared from cadmium-treated HeLa cells, we isolated clones representing mRNAs whose concentration is increased after cadmium induction. Several metallothionein cDNA clones were isolated by cross-hybridization to mouse metallothionein-I cDNA. The nucleotide sequence of one of these clones, containing a nearly full-length cDNA copy of human metallothionein-II mRNA, was determined. The homology between the human and mouse metallothionein sequences is strictly limited to the coding region of the mRNA. Codon usage in metallothionein mRNA is not random. Seventy-nine percent of the codons have G or C residues at the third position, resulting in a GC-rich sequence.