The RABIT: a rapid automated biodosimetry tool for radiological triage.
The RABIT: a rapid automated biodosimetry tool for radiological triage.
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DOI：
10.1097/hp.0b013e3181ab3cb6
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发表时间：
2010-02
期刊：
影响因子：
2.2
通讯作者：
Brenner DJ
中科院分区：
文献类型：
--
作者：
Garty G;Chen Y;Salerno A;Turner H;Zhang J;Lyulko O;Bertucci A;Xu Y;Wang H;Simaan N;Randers-Pehrson G;Yao YL;Amundson SA;Brenner DJ
为了满足人们对大规模放射性事件后使用的高通量生物剂量测定方法的需求，一种合乎逻辑的方法是使目前手动进行的标准生物剂量测定完全自动化。我们描述了RABIT（快速自动生物剂量测定工具）的最新进展，该工具旨在对来自手指针刺单滴血的淋巴细胞中的微核或γ-H2 AX荧光进行评分。RABIT系统设计为完全自动化，从毛细血管血液样本输入到机器，到剂量估计的输出。通过使用单滴血、优化多孔板中原位分析的生物学方案、实施机器人板和液体处理以及高速成像的新发展，实现了通量的提高。自动化完善的生物测定代表了高通量辐射生物剂量测定的一种有前途的方法，这既是因为可以实现高通量，也是因为部署时间可能比新的生物测定短得多。在这里，我们描述的RABIT系统的每个单独的模块的发展，并显示从关键模块的初步数据。正在进行的是系统集成，然后是校准和验证。
In response to the recognized need for high throughput biodosimetry methods for use after large scale radiological events, a logical approach is complete automation of standard biodosimetric assays that are currently performed manually. We describe progress to date on the RABIT (Rapid Automated BIodosimetry Tool), designed to score micronuclei or γ-H2AX fluorescence in lymphocytes derived from a single drop of blood from a fingerstick. The RABIT system is designed to be completely automated, from the input of the capillary blood sample into the machine, to the output of a dose estimate. Improvements in throughput are achieved through use of a single drop of blood, optimization of the biological protocols for in-situ analysis in multi-well plates, implementation of robotic plate and liquid handling, and new developments in high-speed imaging. Automating well-established bioassays represents a promising approach to high-throughput radiation biodosimetry, both because high throughputs can be achieved, but also because the time to deployment is potentially much shorter than for a new biological assay. Here we describe the development of each of the individual modules of the RABIT system, and show preliminary data from key modules. Ongoing is system integration, followed by calibration and validation.