Noninvasive Imaging of Colitis Using Multispectral Optoacoustic Tomography
Noninvasive Imaging of Colitis Using Multispectral Optoacoustic Tomography
复制标题
DOI：
10.2967/jnumed.116.184705
复制
发表时间：
2017-06-01
影响因子：
9.3
通讯作者：
McNally, Lacey R.
中科院分区：
文献类型：
--
作者：
Bhutiani, Neal;Grizzle, William E.;McNally, Lacey R.
目前，正在研究几种非侵入性方式，包括MRI和PET，以识别早期肠道炎症，纵向监测疾病状态，或检测炎症性肠病患者的发育异常变化。在这里，我们评估的适用性和效用的多光谱光声断层扫描（MSOT）在评估结肠炎的存在和严重程度。方法：C57 B/6小鼠未经处理或用脆弱拟杆菌和抗生素介导的肠道植物群耗竭处理以引发结肠炎。使用MSOT对小鼠成像以检测肠道炎症。用MSOT鉴定的肠道炎症也使用结肠镜检查和组织学证实。结果如下：细菌性结肠炎的小鼠表现出时间相关的肠系膜和结肠血管的增加，平均信号强度的增加，氧合血红蛋白（P = 0.004）的MSOT接种后2天。接种后7 d，这些发现明显更加突出，氧合血红蛋白的平均信号强度增加（P = 0.0002），结肠表面点状血管病变的发展，这与结肠镜检查以及组织学观察到的变化相对应。结论：随着组织穿透深度的提高，MSOT可能成为一种敏感、准确、无创的成像工具，用于评价炎症性肠病患者。
Currently, several noninvasive modalities, including MRI and PET, are being investigated to identify early intestinal inflammation, longitudinally monitor disease status, or detect dysplastic changes in patients with inflammatory bowel disease. Here, we assess the applicability and utility of multispectral optoacoustic tomography (MSOT) in evaluating the presence and severity of colitis. Methods: C57B/6 mice were untreated or treated with Bacteroides fragilis and antibiotic- mediated depletion of intestinal flora to initiate colitis. Mice were imaged using MSOT to detect intestinal inflammation. Intestinal inflammation identified with MSOT was also confirmed using both colonoscopy and histology. Results: Mice with bacterial colitis demonstrated a temporally associated increase in mesenteric and colonic vascularity with an increase in mean signal intensity of oxygenated hemoglobin (P = 0.004) by MSOT 2 d after inoculation. These findings were significantly more prominent 7 d after inoculation, with increased mean signal intensity of oxygenated hemoglobin (P = 0.0002) and the development of punctate vascular lesions on the colonic surface, which corresponded to changes observed on colonoscopy as well as histology. Conclusion: With improvements in depth of tissue penetration, MSOT may hold potential as a sensitive, accurate, noninvasive imaging tool in the evaluation of patients with inflammatory bowel disease.