Sigma factor genes sigC, sipE, and sigG are upregulated in heterocysts of the cyanobacterium Anabaena sp strain PCC 7120
Sigma factor genes sigC, sipE, and sigG are upregulated in heterocysts of the cyanobacterium Anabaena sp strain PCC 7120
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DOI：
10.1128/jb.00821-07
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发表时间：
2007-11-01
影响因子：
3.2
通讯作者：
Golden, James W.
中科院分区：
文献类型：
--
作者：
Aldea, M. Ramona;Mella-Herrera, Rodrigo A.;Golden, James W.
我们使用绿色荧光蛋白转录融合，以研究在蓝藻鱼腥藻属菌株PCC 7120的异形胞发育过程中的八个西格玛因子基因的调节。报告菌株含有gfp与sigB2，sigD，sigI和sigJ的上游区域的融合没有表现出发育调控。sigC，sigE，和sigG报告菌株的延时显微镜显示增加的绿色荧光蛋白荧光在分化的细胞在4小时，16小时，和9小时后，氮逐步下降。
We used gfp transcriptional fusions to investigate the regulation of eight sigma factor genes during heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120. Reporter strains containing gfp fusions with the upstream regions of sigB2, sigD, sigI, and sigJ did not show developmental regulation. Time-lapse microscopy of sigC, sigE, and sigG reporter strains showed increased green fluorescent protein fluorescence in differentiating cells at 4 h, 16 h, and 9 h, respectively, after nitrogen step down.