A comparison in individual murine tumors of techniques for measuring oxygen levels
A comparison in individual murine tumors of techniques for measuring oxygen levels
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DOI：
10.1016/s0360-3016(99)00104-2
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发表时间：
1999-07-15
影响因子：
7
通讯作者：
Hill, RP
中科院分区：
文献类型：
--
作者：
Kavanagh, MC;Tsang, V;Hill, RP
目的：为了探讨不同的技术之间的关系，用于测量氧水平在小鼠肿瘤model.Methods和材料：使用小鼠纤维肉瘤KHT-C，五种技术测量氧水平Eppendorf pO（2），Histograph，EFS结合，彗星试验，配对生存试验，并在体内生长延迟试验进行了评估。在这些实验中，三个或更多的技术应用在不同的组合，以测量在个别tumors.Results的氧水平：统计学显着的相关性，观察到从配对的生存分析与那些从EF 5结合计算的缺氧比例之间。发现彗星试验与配对存活分析和生长延迟分析具有统计学显著相关性。Eppendorf pO（2）、Histograph测量值与其他技术测量值之间无统计学显著相关性，尽管与配对存活测定和EF 5结合存在弱相关性。由于技术原因，EF 5结合和生长延迟试验之间没有进行比较，结论：EF 5结合和彗星试验与放射生物学试验之间的相关性表明，这些技术有可能预测放射治疗后的结果。在pO（2）、组织学数据和放射生物学测定之间缺乏相关性，这与早期临床试验的结果相反。(C)1999 Elsevier Science Inc.
Purpose: To investigate the relationship between different techniques for measuring oxygen levels in a murine tumor model.Methods and Materials: Using the murine fibrosarcoma line KHT-C, five techniques of measuring oxygen levels-the Eppendorf pO(2), Histograph, EFS binding, the comet assay, a paired survival assay, and an in vivo growth delay assay-were assessed. In these experiments, three or more techniques were applied in different combinations to measure the oxygen levels in individual tumors.Results: Statistically significant correlations were observed between the hypoxic proportions calculated from the paired survival assay with those from EF5 binding. The comet assay was found to have a statistically significant correlation with the paired survival analysis and the growth delay analysis. No statistically significant correlation was found between the Eppendorf pO(2), Histograph measurements and those from the other techniques, although there were weak correlations with the paired survival assay and EF5 binding. For technical reasons, a comparison was not made between EF5 binding and the growth delay assay,Conclusions: The correlations found between EF5 binding and the comet assay with the radiobiological assays suggest that these techniques have potential for predicting outcome following radiation treatment. The lack of correlation seen between the pO(2), Histograph data and the radiobiological assays is in contrast to results from early clinical trials. (C) 1999 Elsevier Science Inc.