Identification of RAPD markers linked to A and B genome sequences in Musa L.
Identification of RAPD markers linked to A and B genome sequences in Musa L.
复制标题
鉴定与香蕉 A 和 B 基因组序列相关的 RAPD 标记。
作者：
M. Pillay;D. Nwakanma;A. Tenkouano
大蕉和香蕉（Musa spp. sect. eumusa）起源于两种野生二倍体物种 M. acuminata Colla 之间的种内和种间杂交。和 M. balbisiana Colla.，分别贡献了 A 和 B 基因组。多倍体和杂交产生了许多具有A和B基因组不同排列的二倍体、三倍体和四倍体克隆。因此，甜点香蕉和高地香蕉主要被分类为 AAA，大蕉被分类为 AAB，烹饪香蕉被分类为 ABB。芭蕉属基因组的分类是基于形态特征的。本研究旨在鉴定 A 和 B 基因组的 RAPD（随机扩增多态性 DNA）标记。使用 80 个 10 聚体操纵子引物来扩增来自 M. acuminata 亚种的 DNA。 burmannicoides 克隆“加尔各答 4”（AA 基因组）和 M. balbisiana 克隆“洪都拉斯”（BB 基因组）。鉴定出在这两个物种中产生独特的基因组特异性片段的三个引物（A17、A18 和 D10）。这些引物在代表各种基因组组合的 40 种基因型样本中进行了测试。 RAPD 标记能够阐明所有基因型的基因组组成。结果表明，RAPD 分析可以为香蕉基因组鉴定提供快速可靠的系统，从而促进育种系中的基因组表征和操作。
Plantains and bananas (Musa spp. sect. eumusa) originated from intra- and interspecific hybridization between two wild diploid species, M. acuminata Colla. and M. balbisiana Colla., which contributed the A and B genomes, respectively. Polyploidy and hybridization have given rise to a number of diploid, triploid, and tetraploid clones with different permutations of the A and B genomes. Thus, dessert and highland bananas are classified mainly as AAA, plantains are AAB, and cooking bananas are ABB. Classification of Musa into genomic groups has been based on morphological characteristics. This study aimed to identify RAPD (random amplified polymorphic DNA) markers for the A and B genomes. Eighty 10-mer Operon primers were used to amplify DNA from M. acuminata subsp. burmannicoides clone 'Calcutta 4' (AA genomes) and M. balbisiana clone 'Honduras' (BB genomes). Three primers (A17, A18, and D10) that produced unique genome-specific fragments in the two species were identified. These primers were tested in a sample of 40 genotypes representing various genome combinations. The RAPD markers were able to elucidate the genome composition of all the genotypes. The results showed that RAPD analysis can provide a quick and reliable system for genome identification in Musa that could facilitate genome characterization and manipulations in breeding lines.