Blood microRNA-93 as an indicator for diagnosis and prediction of functional recovery of acute stroke patients
Blood microRNA-93 as an indicator for diagnosis and prediction of functional recovery of acute stroke patients
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血液microRNA-93作为诊断和预测急性脑卒中患者功能恢复的指标。
DOI：
10.1016/j.jocn.2018.12.003
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发表时间：
2019-04-01
影响因子：
2
通讯作者：
Zhao, Haiping
中科院分区：
文献类型：
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作者：
Ma, Qingfeng;Li, Guangwen;Zhao, Haiping
本研究评估了microRNA-93在急性缺血性卒中（AIS）患者中的诊断和预测潜力，以及其在体外对小胶质细胞炎症的调节。结果显示，AIS患者血浆和中性粒细胞中的miR-93水平均明显降低，Pearson相关分析显示血浆和中性粒细胞中的miR-93水平存在显著的线性正相关。此外，脑卒中患者入院时血浆和中性粒细胞中的miR-93水平与梗死体积和入院时NIHSS（美国国立卫生研究院卒中量表）评分无关，但中性粒细胞miR-93水平与卒中后7天的Barthel指数呈正相关。重要的是，AIS患者血浆和中性粒细胞中的miR-93水平与TNF-α和IL-10的表达呈负相关。此外，通过流式细胞术测试，用miR-93阿戈米尔体外处理降低了OGD（氧和葡萄糖剥夺）诱导的BV 2小胶质细胞增殖。我们证明，血液中的miR-93有可能促进急性缺血性卒中的神经学结局的诊断和预测，并且可能通过靶向小胶质细胞的增殖参与炎症。(C)2018爱思唯尔有限公司版权所有
The present study evaluated the diagnostic and predictive potential of microRNA-93 in acute ischemic stroke (AIS) patients within 6 h of stroke onset and its regulation on microglial inflammation in vitro. Our results showed that the miR-93 levels in plasma and neutrophil detected by real-time PCR were evidently reduced in AIS patients, and Pearson's correlation analysis showed that miR-93 levels in plasma and neutrophils had a significant positive linear correlation. Moreover, miR-93 levels in plasma and neutrophils of stroke patients at time of admission were not correlated with infarct volume and NIHSS (National Institute of Health stroke scale) scores at admission, but neutrophil miR-93 levels were positively correlated with the Barthel Index 7 days after stroke. Importantly, miR-93 levels in plasma and neutrophil of AIS patients were negatively correlated with the expression of TNF-alpha and IL-10. Furthermore, in vitro treatment with miR-93 agomir decreased the OGD (Oxygen and glucose deprivation)-induced proliferation of BV2 microglial cells tested by Flow cytometry. We demonstrated that miR-93 in blood has a potential to facilitate the diagnosis and prediction of neurological outcomes of acute ischemic stroke, and is involved in inflammation possibly through targeting the proliferation of microglia. (C) 2018 Elsevier Ltd. All rights reserved.