Entomological evaluation by pool screen polymerase chain reaction of Onchocerca volvulus transmission in Ecuador following mass Mectizan™ distribution
Entomological evaluation by pool screen polymerase chain reaction of Onchocerca volvulus transmission in Ecuador following mass Mectizan™ distribution
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DOI：
10.4269/ajtmh.2003.68.222
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发表时间：
2003-02-01
影响因子：
3.3
通讯作者：
Unnasch, TR
中科院分区：
文献类型：
--
作者：
Guevara, AG;Vieira, JC;Unnasch, TR
在厄瓜多尔流行盘尾丝虫病的三个流域，通过池筛选聚合酶链反应（PCR）扩增估计感染和感染性黑蝇的患病率。伊维菌素（Mectizan（TM））的大规模分发导致受感染和感染性苍蝇的流行率急剧下降。在里约圣地亚哥河流域，没有检测到感染，表明传播已经停止。感染的比例比四带蚋低10倍，表明S。exiguum比S. quadrivittatum。然而，两种蝇类感染性的流行率没有差异，这表明S。quadrivittatum可能发挥重要的媒介作用，它是主要的人类叮咬物种。这些数据表明，池筛选PCR是监测受控制地区传播情况和证明一个地区无O。肠扭转传播
The prevalence of infected and infective black flies was estimated by pool screen polymerase chain reaction (PCR) amplification in the three river basins in Ecuador endemic for onchocerciasis. Mass distribution of ivermectin (Mectizan(TM)) resulted in dramatic declines in the prevalence of infected and infective flies. In the Rio Santiago river basin, no infections were detected, suggesting that transmission had ceased. The ratio of infected to infective flies in Simulium exiguum was 10-fold lower than the corresponding ratio for Simulium quadrivittatum, suggesting that S. exiguum is a more-competent vector for Onchocerca volvulus than S. quadrivittatum. However, the prevalence of infective flies in the two species was not different, suggesting that S. quadrivittatum may play an important vectorial role where it is the dominant human-biting species. The data demonstrate that pool screen PCR is an efficient way to monitor transmission in areas subject to control, and to certify an area as free of O. volvulus transmission.