Single-nucleotide polymorphism data support the general unrelatedness of the males in the agricultural health study.
Single-nucleotide polymorphism data support the general unrelatedness of the males in the agricultural health study.
复制标题
单核苷酸多态性数据支持农业健康研究中男性普遍不相关的观点。
DOI：
10.1158/1055-9965.epi-14-0276
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Hoppin,JaneA
中科院分区：
文献类型：
--
作者：
Jack,JohnR;Motsinger-Reif,AlisonA;Koutros,Stella;Alavanja,MichaelC;BeaneFreeman,LauraE;Hoppin,JaneA
背景：农业通常是一个家庭和几代人的生意。农民之间的亲缘关系可能会影响基因-环境相互作用的分析。为了评估农民的潜在相关性，我们使用了农业健康研究（AHS）中进行的前列腺癌巢式病例对照研究的数据，这是一项对爱荷华州和北卡罗来纳州农民的前瞻性研究。方法：我们分析了来自2220名白人参与者的25009个snp（单核苷酸多态性）的遗传数据，以测试这些农民之间的隐性亲缘关系。我们使用了两个软件包：(i) PLINK，用于计算近交系数和血统识别（IBD）统计，（ii） EIGENSOFT，用于对遗传数据进行主成分分析。结果：近交系数估计值和IBD统计数据显示，受试者绝大多数不相关，这些数据中几乎没有潜在的隐性相关性。结论：我们的分析否定了病例对照研究中个体表现出隐性亲缘关系的假设。影响：这些发现对AHS中基因-环境相互作用的所有后续分析都很重要。癌症流行病学生物标志物；23日(10);2192 - 5。AACR©2014。
Background:Farming is often a family and multigenerational business. Relatedness among farmers could bias gene–environment interaction analysis. To evaluate the potential relatedness of farmers, we used data from a nested case–control study of prostate cancer conducted in the Agricultural Health Study (AHS), a prospective study of farmers in Iowa and North Carolina.Methods:We analyzed the genetic data for 25,009 SNPs (single-nucleotide polymorphisms) from 2,220 White participants to test for cryptic relatedness among these farmers. We used two software packages: (i) PLINK, to calculate inbreeding coefficients and identity-by-descent (IBD) statistics and (ii) EIGENSOFT, to perform a principal component analysis on the genetic data.Results:Inbreeding coefficients estimates and IBD statistics show that the subjects are overwhelmingly unrelated, with little potential for cryptic relatedness in these data.Conclusions:Our analysis rejects the hypothesis that individuals in the case–control study exhibit cryptic relatedness.Impact:These findings are important for all subsequent analyses of gene–environment interactions in the AHS.Cancer Epidemiol Biomarkers Prev; 23(10); 2192–5. ©2014 AACR.