Expression in Escherichia coli of Multiple Products from a Chimaeric Gene Fusion: Evidence for the Presence of Procaryotic Translational Control Regions within Eucaryotic Genes
Expression in Escherichia coli of Multiple Products from a Chimaeric Gene Fusion: Evidence for the Presence of Procaryotic Translational Control Regions within Eucaryotic Genes
复制标题
嵌合基因融合多种产物在大肠杆菌中的表达：真核基因中存在原核翻译控制区的证据
DOI：
10.1038/nbt0885-715
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Susan Smith
中科院分区：
文献类型：
--
作者：
S. Halling;Susan Smith
Expression in Escherichia coli of a chimaeric gene fusion containing a porcine parvovirus (PPV) DNA fragment produced more than one size fusion protein. The introduction of a translation termination codon between the initiation codon and the PPV sequences of the gene fusion did not prevent the expression of the smaller fusion proteins. This suggests that the smaller products are the result of independent initiations of translation within PPV sequences rather than proteolysis of the full-length fusion protein. Three translation initiation sites were mapped to different segments of PPV DNA by protein sizing and deletion analysis. The sequences of these segments are presented.
影响因子：
3.5
作者：
DAVIS, AR;BOS, T;COMPANS, RW
通讯作者：
COMPANS, RW