Lipid-Controlled Stabilization of Charge-Separated States (P+QB?) and Photocurrent Generation Activity of a Light-Harvesting?Reaction Center Core Complex (LH1-RC) from Rhodopseudomonas palustris
Lipid-Controlled Stabilization of Charge-Separated States (P+QB?) and Photocurrent Generation Activity of a Light-Harvesting?Reaction Center Core Complex (LH1-RC) from Rhodopseudomonas palustris
复制标题
脂质控制的电荷分离态稳定 (P QB?) 和光捕获反应中心核心复合物 (LH1-RC) 的光电流产生活性
DOI：
10.1021/acs.jpcb.7b09973
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Dewa Takehisa
中科院分区：
文献类型：
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作者：
Noji Tomoyasu;Matsuo Mikano;Takeda Nobutaka;Sumino Ayumi;Kondo Masaharu;Nango Mamoru;Itoh Shigeru;Dewa Takehisa
光合捕光反应中心核心复合物（LH 1-RC）是一种天然的激子和光伏器件，嵌入在脂质膜中。为了将LH 1-RCs作为生物混合能源生产材料应用，必须解决一些重要问题，包括如何使LH 1-RCs尽可能有效地发挥作用。此外，它们应该被表征以评估有多少活性LH 1-RC在人工系统中有效地工作。我们在这里报告，一种阴离子磷脂，磷脂酰甘油（PG），稳定的电荷分离状态（光氧化的电子供体和还原醌对，P+QB-）的LH 1-RC（从Rhodoptera palustris），并提高其在光电流产生的活性。稳态荧光分析表明，PG增强的P+ QB-状态的形成在较低的辐照度。通过Michaelis-Menten动力学分析了光电流产生活性，揭示了38%的LH 1-RC重组到PG膜中以46 s-1的转换频率产生光电流。PG分子在体内与LH 1-RC相互作用，作为LH 1-RC的活性效应成分，增强其在生物杂交系统中的功能。
The photosynthetic light-harvesting–reaction center core complex (LH1-RC) is a natural excitonic and photovoltaic device embedded in a lipid membrane. In order to apply LH1-RCs as a biohybrid energy-producing material, some important issues must be addressed, including how to make LH1-RCs function as efficiently as possible. In addition, they should be characterized to evaluate how many active LH1-RCs efficiently work in artificial systems. We report here that an anionic phospholipid, phosphatidylglycerol (PG), stabilizes the charge-separated state (a photooxidized electron donor and reduced quinone pair, P+QB–) of LH1-RC (fromRhodopseudomonas palustris) and enhances its activity in photocurrent generation. Steady-state fluorometric analysis demonstrated that PG enhances the formation of the P+QB–state at lower irradiances. The photocurrent generation activity was analyzed via Michaelis–Menten kinetics, revealing that 38% of LH1-RCs reconstituted into the PG membrane generated photocurrent at a turnover frequency of 46 s–1. PG molecules, which interact with LH1-RC in vivo, play the role of an active effector component for LH1-RC to enhance its function in the biohybrid system.