An NMR investigation of the binding of the anticancer drug actinomycin D to oligodeoxyribonucleotides with isolated 5'd(GC)3' binding sites.
An NMR investigation of the binding of the anticancer drug actinomycin D to oligodeoxyribonucleotides with isolated 5'd(GC)3' binding sites.
复制标题
抗癌药物放线菌素 D 与具有分离的 5d(GC)3 结合位点的寡脱氧核糖核苷酸结合的 NMR 研究。
DOI：
10.1021/bi00416a029
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发表时间：
1988
期刊：
影响因子：
2.9
通讯作者：
Wilson,WD
中科院分区：
文献类型：
--
作者：
Jones,RL;Scott,EV;Zon,G;Marzilli,LG;Wilson,WD
Materials and MethodsMaterials. The spectrum of ActD (Sigma) agreed with literature results (Brown et al., 1984), and the compound was used without further purification. ActD stock solutions were made by dissolving ActD in water to a final concentration of approximately 1 mM. Finalconcentrations were determined by using an extinction coefficient of 24 500 M" 1 cm" 1 at 440 nm (Shafer et al., 1980). PIPES 10 buffer (10 mM PIPES,
