The role of cell bioaugmentation and gene bioaugmentation in the remediation of co-contaminated soils.
The role of cell bioaugmentation and gene bioaugmentation in the remediation of co-contaminated soils.
复制标题
细胞生物学和基因生物学在共污染土壤修复中的作用。
DOI：
10.1289/ehp.02110s6943
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发表时间：
2002-12
影响因子：
10.4
通讯作者：
Josephson KL
中科院分区：
文献类型：
--
作者：
Pepper IL;Gentry TJ;Newby DT;Roane TM;Josephson KL
被金属和有机物共污染的土壤给修复带来了特殊的问题。金属污染可以延缓或抑制微生物对有机污染物的降解，因此为了有效地原位生物降解，生物强化是必要的。我们监测了2，4-二氯苯氧乙酸(2，4-D)或3-氯苯甲酸酯(3-CB)在镉(Cd)污染和不污染(Cd)两种不同土壤中的降解情况。此外，我们评估了生物强化在这些共污染土壤中促进有机降解的能力。最后，我们确定加速降解是由于引入的生物存活(细胞生物增强)还是由于质粒转移到本地微生物群体(基因生物增强)。在巴西土壤中，一株抗Cd细菌和一株已知的2，4-D降解菌JMP134的双重接种促进了2，4-D的降解。利用缺乏2，4-D完全矿化所必需的染色体基因的大肠杆菌D11在Madera土壤中进行基因生物增强。与对照相比，2，4-D的降解率提高。用睾酮单胞菌促进3-CB在Madera土壤中的生物降解，进一步证明了细胞的生物强化作用。在这种情况下，没有观察到质粒pBRC60转移到本地土壤受体。对于Madera土壤，非生物强化样品最终显示出完全的2，4-D降解。相比之下，非生物强化的布拉西托土壤表现出不完全的2，4-D降解。这些研究的独特之处在于，表明细胞生物增强和基因生物增强都可以有效地促进共污染土壤中的有机降解。归根结底，生物强化战略可能取决于污染程度和可用于补救的时间框架。
Soils co-contaminated with metals and organics present special problems for remediation. Metal contamination can delay or inhibit microbial degradation of organic pollutants such that for effective in situ biodegradation, bioaugmentation is necessary. We monitored the degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) or 3-chlorobenzoate (3-CB) in two different soils with and without cadmium (Cd) contamination. Additionally, we evaluated the ability of bioaugmentation to enhance organic degradation in these co-contaminated soils. Finally, we determined whether enhanced degradation was due to survival of the introduced organism (cell bioaugmentation) or plasmid transfer to indigenous microbial populations (gene bioaugmentation). In Brazito soil, dual inoculation with a Cd-resistant bacterium plus a known 2,4-D-degrading bacterium, Ralstonia eutropha JMP134, enhanced 2,4-D degradation. Escherichia coli D11, which lacks chromosomal genes necessary for complete 2,4-D mineralization, was used for gene bioaugmentation in Madera soil. Significant gene transfer of the plasmid to the indigenous populations was observed, and the rate of 2,4-D degradation was enhanced relative to that of controls. Cell bioaugmentation was further demonstrated when (Comamonas testosteroni was used to enhance biodegradation of 3-CB in Madera soil. In this case no transfer of plasmid pBRC60 to indigenous soil recipients was observed. For the Madera soil, nonbioaugmented samples ultimately showed complete 2,4-D degradation. In contrast, nonbioaugmented Brazito soils showed incomplete 2,4-D degradation. These studies are unique in showing that both cell bioaugmentation and gene bioaugmentation can be effective in enhancing organic degradation in co-contaminated soils. Ultimately, the bioaugmentation strategy may depend on the degree of contamination and the time frame available for remediation.
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影响因子：
14.9
作者：
VERSALOVIC, J;KOEUTH, T;LUPSKI, JR
通讯作者：
LUPSKI, JR
影响因子：
3.5
作者：
Nakatsu, CH;Providenti, M;Wyndham, RC
通讯作者：
Wyndham, RC
影响因子：
2.9
作者：
BAATH, E
通讯作者：
BAATH, E
影响因子：
4.4
作者：
Newby, DT;Gentry, TJ;Pepper, IL
通讯作者：
Pepper, IL
影响因子：
4.4
作者：
NEILSON, JW;JOSEPHSON, KL;SINCLAIR, NA
通讯作者：
SINCLAIR, NA