Electrostatic field-induced tip-electrospray ionization mass spectrometry for direct analysis of raw food materials
Electrostatic field-induced tip-electrospray ionization mass spectrometry for direct analysis of raw food materials
复制标题
静电场诱导尖端电喷雾电离质谱法用于直接分析食品原料
DOI：
10.1002/jms.4309
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发表时间：
2019
影响因子：
2.3
通讯作者：
Hu Bin
中科院分区：
文献类型：
--
作者：
Li Lei;Li Wen;Hu Bin
快速鉴定蔬菜、肉类和可食用动植物等原料中的代谢物和危险化合物，如化学残留物和天然毒素，对确保食品质量和安全具有重要作用。为了快速分析食品原料中的分析物，有必要建立直接分析食品原料的原位分析方法。在这项工作中，包括生物组织和活体样品在内的食品原料被放置在电极和质谱仪(MS)入口之间，在强大的静电场下，通过在样品上加入一滴溶剂，分析物被快速诱导从样品尖端产生电喷雾电离(ESI)。因此，静电场诱导的电喷雾电喷雾串联质谱仪(TIP-ESI-MS)可以避免原料样品接触高压，因此该方法在食品活体动植物分析中具有优势。已成功检测到来自食品原料的代谢物分析、农药和兽药残留以及天然毒素。考察了直接样品分析的线性范围、灵敏度和重现性等分析性能。文中还讨论了静电场诱导的针尖ESI的电离机理。
Rapid characterization of metabolites and risk compounds such as chemical residues and natural toxins in raw food materials such as vegetables, meats, and edible living plants and animals plays an important part in ensuing food quality and safety. To rapidly characterize the analytes in raw food materials, it is essential to develop in situ method for directly analyzing raw food materials. In this work, raw food materials including biological tissues and living samples were placed between an electrode and mass spectrometric (MS) inlet under a strong electrostatic field; analytes were rapidly induced to generate electrospray ionization (ESI) from the sample tip by adding a drop of solvent onto the sample. Therefore, the electrostatic field–induced tip‐ESI‐MS allows raw samples to avoid contacting high voltage, and thus this method has the advantage for in vivo analysis of food living plants and animals. Metabolite profiling, residues of pesticides and veterinary drugs, and natural toxins from raw food materials have been successfully detected. The analytical performances, including the linear ranges, sensitivity, and reproducibility, were investigated for direct sample analysis. The ionization mechanism of electrostatic field–induced tip‐ESI was also discussed in this work.