Identification and characterization of 17β-hydroxysteroid dehydrogenases in the zebrafish, Danio rerio
Identification and characterization of 17β-hydroxysteroid dehydrogenases in the zebrafish, Danio rerio
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DOI：
10.1016/j.mce.2003.11.010
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发表时间：
2004-02-27
影响因子：
4.1
通讯作者：
Adamski, J
中科院分区：
文献类型：
--
作者：
Mindnich, R;Deluca, D;Adamski, J
17 β-羟基类固醇脱氢酶（17 β-HSDs）是类固醇激素合成最后步骤中的关键酶。17 β-HSD 1型（HSD 17 B1）催化雌酮还原为雌二醇，而3型（HSD 17 B3）执行雄烯二酮转化为睾酮。在这里，我们提出了一个功能基因组学研究这些酶在斑马鱼的假定候选人。通过对斑马鱼EST数据库的计算机筛选，我们鉴定了HSD 17 B1和HSD 17 B3的三个候选同源物。系统发育分析，独特的表达模式（RT-PCR）在胚胎发育和成年期，以及活动测量显示，其中一个HSD 17 B1候选人是斑马鱼同源物，而其他两个是旁系同源的光感受器相关的视黄醇脱氢酶。所有三个HSD 17 B3候选基因在胚胎发生和成体组织中表现出几乎相同的普遍表达，并且被鉴定为HSD 17 B12的旁系同源物和尚未表征的推定类固醇脱氢酶。系统发育分析表明，HSD 17 B3和HSD 17 B12是来自一个共同祖先的后代。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
The 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) are key enzymes in the final steps of steroid hormone synthesis. 17beta-HSD type 1 (HSD17B1) catalyzes the reduction of estrone to estradiol, while type 3 (HSD17B3) performs the conversion of androstenedione to testosterone. Here we present a functional genomics study of putative candidates of these enzymes in the zebrafish. By an in silico screen of zebrafish EST databases we identified three candidate homologs for both HSD17B1 and HSD 17B3. Phylogenetic analysis, unique expression patterns (RT-PCR) during etnbryogenesis and adulthood, as well as activity measurements revealed that one of the HSD17B1 candidates is the zebrafish homolog, while the other two are paralogous photoreceptor-associated retinol dehydrogenases. All three HSD17B3 candidate genes showed nearly identical, ubiquitous expressions in embryogenesis and adult tissues and were identified to be paralogs of HSD17B12 and a yet uncharacterized putative steroid dehydrogenase. Phylogenetic analysis shows that HSD17B3 and HSD17B12 are descendants from a common ancestor. (C) 2003 Elsevier Ireland Ltd. All rights reserved.