Cholesterol levels in plasma and cerebrospinal fluid in patients with clinically isolated syndrome and relapsing-remitting multiple sclerosis
Cholesterol levels in plasma and cerebrospinal fluid in patients with clinically isolated syndrome and relapsing-remitting multiple sclerosis
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DOI：
10.1016/j.nbd.2022.105889
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发表时间：
2022-10-08
影响因子：
6.1
通讯作者：
Markianos, Manolis
中科院分区：
文献类型：
--
作者：
Evangelopoulos, Maria-Eleftheria;Koutsis, Georgios;Markianos, Manolis
胆固醇是髓鞘形成所必需的，但也可以调节适应性免疫反应的机制。目前尚不清楚是否缺乏髓鞘再生的多发性硬化症（MS）的结果，从少突胶质细胞前体细胞分化能力不足或胆固醇不足。一些研究已经评估了脂质谱及其代谢与脱髓鞘、残疾和疾病进展的潜在关联。为探讨复发缓解型多发性硬化（RRMS）和临床孤立综合征（CIS）患者血浆和脑脊液（CSF）中胆固醇水平与疾病特征的关系，对62例CIS和46例RRMS患者进行了研究。所有患者的EDSS均较低，并且在评估时均未使用药物。48名年龄相同的受试者作为对照。采用酶比色法测定血浆和脑脊液胆固醇浓度，并与临床状态、磁共振成像（MRI）中疾病活动性和脑脊液中寡克隆带（OBs）的存在相关。与OBs阴性患者相比，OBs患者的CSF胆固醇水平显着降低，但血浆胆固醇水平不降低。血浆胆固醇水平与年龄呈正相关，脑脊液胆固醇水平与EDSS呈正相关。我们的结果表明，MS患者中OBs阳性的CSF胆固醇水平低可能反映了广泛的脱髓鞘和更严重的疾病过程，与抗膜成分的体液免疫反应增强有关。
Cholesterol is essential for myelin formation, but may also modulate mechanisms involved in adaptive immune responses. It is unclear whether lack of remyelination in multiple sclerosis (MS) results from an insufficient capacity of oligodendrocyte precursor cells to differentiate or from cholesterol insufficiency. Several studies have assessed the potential association of lipid profile and its metabolism with demyelination, disability, and disease progression. The aim of the present study was to measure cholesterol levels in plasma and cerebrospinal fluid (CSF) of patients with relapsing remitting MS (RRMS) or with clinically isolated syndrome (CIS), and to inves-tigate whether there is an association between cholesterol levels and disease characteristics.Sixty-two patients with CIS and 46 patients with RRMS were included in the study. All patients had low EDSS and were medication-free at assessment. Forty-eight subjects within the same age range served as controls. Cholesterol concentrations were measured in plasma and in CSF by the same enzymatic - colorimetric method, and were related to clinical status, disease activity in magnetic resonance imaging (MRI) and presence of oli-goclonal bands in CSF (OBs).Significantly lower levels of plasma and CSF cholesterol were found in patients compared to controls. Patients with OBs showed significantly lower levels of CSF cholesterol but not plasma cholesterol compared to OBs-negative patients. A positive correlation of plasma cholesterol with age and of CSF cholesterol with EDSS was found.Our results suggest that low CSF cholesterol in MS patients with positive OBs might reflect extensive demy-elination and a more aggressive disease course associated with an increased humoral immune response against membrane components.