Protective effect of quercetin on hyperglycemia, oxidative stress and DNA damage in alloxan induced type 2 diabetic mice
Protective effect of quercetin on hyperglycemia, oxidative stress and DNA damage in alloxan induced type 2 diabetic mice
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DOI：
10.1016/j.lfs.2014.06.005
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发表时间：
2014-07-25
期刊：
影响因子：
6.1
通讯作者：
Naseem, Imrana
中科院分区：
文献类型：
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作者：
Alam, Md. Maroof;Meerza, Dilnasheen;Naseem, Imrana
目的：槲皮素是一种天然的多酚类黄酮类化合物，可作为任何物理或化学作用产生的活性氧的猝灭剂。2型糖尿病(T2 DM)的基本特征是高血糖，这会导致氧化应激的并发症。鉴于此，本研究旨在探讨槲皮素对2型糖尿病小鼠的影响。主要方法：18只小鼠随机分为对照组、糖尿病组和糖尿病组。测定空腹血糖(FBG)和抗氧化物酶活性。关键发现：与糖尿病组相比，治疗组的空腹血糖水平和肝肾标志物酶显著降低。补充槲皮素后，谷胱甘肽、超氧化物歧化酶、过氧化氢酶和谷胱甘肽-S转移酶水平也增加。治疗组硫代巴比妥酸反应物水平降低，GLUT4表达水平升高。单细胞碱性凝胶电泳法显示，槲皮素对DNA损伤也有正向影响。因此，我们可能提出了一种可能的抗氧化潜力和对T2 DM小鼠的保护作用。意义：从本研究中，我们得出结论：通过钝化自由基对T2 DM小鼠的毒性，具有改善高血糖和氧化应激的作用。(C)2014 Elsevier Inc.保留所有权利。
Aims: Quercetin is a natural polyphenolic flavonoid and acts as a quencher for reactive oxygen species generated by any physical or chemical action. In type 2 diabetes mellitus (T2DM) the basic characteristic feature is hyper-glycemia which leads to complications involving oxidative stress. In view of this, the present study was conducted to examine the effect of quercetin in T2DM.Main methods: A total of 18 mice were divided into three groups, vis control, diabetic and diabetic treated with quercetin. Fasting blood glucose (FBG) levels and anti-oxidant enzyme activity were assayed. Creatinine, urea, lipid peroxidation, GLUT4 expression and DNA damage were also measured.Key findings: A significant decrease in FBG level and liver and kidney marker enzymes was observed in the quercetin treated group as compared to the diabetic one. Glutathione, SOD, catalase, and glutathione-S-transferase levels were also found to be increased on quercetin supplementation. Thiobarbituric acid-reactive substance level was decreased while GLUT4 expression levels were increased in the treated group. DNA damage was also affected positively by quercetin when subjected with single cell alkaline gel electrophoresis. Thus, we may suggest an anti-oxidant potential and protective effect of quercetin in T2DM mice.Significance: From this study, we conclude that quercetin ameliorates hyperglycemia and oxidative stress, by blunting free radical induced toxicity in T2DM. (C) 2014 Elsevier Inc All rights reserved.