DNA METHYLTRANSFERASE GENES OF BACILLUS-SUBTILIS PHAGES - STRUCTURAL RELATEDNESS AND GENE-EXPRESSION
DNA METHYLTRANSFERASE GENES OF BACILLUS-SUBTILIS PHAGES - STRUCTURAL RELATEDNESS AND GENE-EXPRESSION
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DOI：
10.1016/0378-1119(85)90166-0
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发表时间：
1985-01-01
期刊：
影响因子：
3.5
通讯作者：
TRAUTNER, TA
中科院分区：
文献类型：
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作者：
NOYERWEIDNER, M;JENTSCH, S;TRAUTNER, TA
温带B.枯草芽孢杆菌vphi.3T、ρ 11和SP β。在大肠杆菌中克隆表达。每个基因指定一个47千道尔顿的蛋白质，其修饰BsuR（GGCC）和Fnu 4 HI（GCNGC）靶序列。转录由位于克隆片段上的噬菌体启动子控制。确定转录方向和MTase基因的大致位置。DNA/DNA杂交实验揭示了v φ 3 T、ρ 11和SP β的密切结构相关性。基因.在这些基因和相关噬菌体SPR的MTase基因之间也发现了显着程度的同源性，其编码具有不同修饰特异性的酶。这些结果表明不同噬菌体MTase基因的共同祖先。噬菌体Z是该噬菌体群中唯一的BsuR敏感性成员，它缺乏修饰基因，但含有与SPR、vphi 3 T、ρ 11和SP β侧翼序列同源的区域。 MTase基因。
The DNA methyltransferase (Mtase) genes of temperate B. subtilis phages .vphi.3T, .rho.11 and SP.beta. were cloned and expressed in Escherichia coli. Each gene specifies a 47 kilodalton protein, which modifies BsuR (GGCC) and Fnu4HI (GCNGC) target sequences. Transcription is controlled by phage promoters located on the cloned fragments. The direction of transcription and the approximate position of the Mtase genes were determined. DNA/DNA hybridization experiments revealed close structural relatedness of the .vphi.3T, .rho.11 and SP.beta. genes. A significant degree of homology was also found among these genes and the Mtase gene of related phage SPR, which codes for an enzyme with different modification specificity. These results suggest a common ancestor of the different phage Mtase genes. Phage Z, the only BsuR-sensitive member of this phage group, lacks a modification gene, but contains regions homologous to sequences flanking the SPR, .vphi.3T, .rho.11 and SP.beta. Mtase genes.