DISSOCIATION KINETICS OF 19 BASE PAIRED OLIGONUCLEOTIDE-DNA DUPLEXES CONTAINING DIFFERENT SINGLE MISMATCHED BASE-PAIRS
DISSOCIATION KINETICS OF 19 BASE PAIRED OLIGONUCLEOTIDE-DNA DUPLEXES CONTAINING DIFFERENT SINGLE MISMATCHED BASE-PAIRS
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DOI：
10.1093/nar/15.2.797
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发表时间：
1987-01-26
影响因子：
14.9
通讯作者：
ITAKURA, K
中科院分区：
文献类型：
--
作者：
IKUTA, S;TAKAGI, K;ITAKURA, K
本文研究了含有不同单错配碱基对的19个碱基对的寡核苷酸-DNA链在干琼脂糖凝胶上的解离动力学。在我们的实验条件下，解离动力学是一级的。单个错配碱基对的掺入在一定程度上使DNA双链体不稳定，其量取决于错配碱基对的性质。G-T和G-A错配使双链体不稳定，而A-A、T-T、C-T和C-A错配则使双链体不稳定，根据Arrhenius图的斜率，这些含19个碱基对的寡核苷酸-DNA双链体的解离活化能为52 ± 2 Kcalmol− 1。
The dissociation kinetics of 19 base paired oligonucleotide-DNA duplexcontaining a various single mismatched base pair are studied on dried agarose gels. The kinetics of the dissociation are first order under our experimental conditions. The incorporation of a single mismatched base pair destabilizes the DNA duplexes to some extent, the amount depending on the nature of the mismatched base pair. G-T and G-A mismatches slightly destabilize a duplex, while A-A, T-T, C-T and C-A mismatches significantly destabilize it. The activation energy for the overall dissociation processes for these oligonucleotide-DNA duplexes containing 19 base pairs is 52 ± 2 Kcalmol−1as determined from the slope of Arrhenius plot.