Circular RNA alterations in the Bombyx mori midgut following B. mori nucleopolyhedrovirus infection
Circular RNA alterations in the Bombyx mori midgut following B. mori nucleopolyhedrovirus infection
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家蚕核多角体病毒感染后家蚕中肠的环状 RNA 变化
DOI：
10.1016/j.molimm.2018.08.008
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发表时间：
2018-09-01
影响因子：
3.6
通讯作者：
Gong, Chengliang
中科院分区：
文献类型：
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作者：
Hu, Xiaolong;Zhu, Min;Gong, Chengliang
迄今为止，还没有系统的研究检测家蚕感染B.mori核型多角体病毒（BmNPV）后circRNA的表达谱。为了研究家蚕中肠circRNA在BmNPV感染后的表达模式，采用高通量测序技术分析了正常中肠和BmNPV感染后中肠circRNA的表达。共有353个circRNA显著差异表达，其中241个在感染后上调，112个下调。GO注释和KEGG通路分析表明，在BmNPV感染的中肠中富集了许多关键的免疫通路和代谢通路。预测的与circRNA相互作用的miRNAs靶点的潜在作用表明，BmNPV感染显著丰富了泛素、凋亡和内吞信号通路。
Thus far, no systematic studies have examined circRNA expression profiles in the silkworm following B.mori nucleopolyhedrovirus (BmNPV) infection. To explore the expression patterns of circRNAs in the silkworm midgut following BmNPV infection, circRNAs in normal midguts and BmNPV-infected midguts were analyzed by high-throughput sequencing. A total of 353 circRNAs were significantly differentially expressed, of which 241 were upregulated and 112 were downregulated following infection. GO annotation and KEGG pathways analyses of these circRNAs showed that many key immunity pathways and metabolism pathways were enriched in the BmNPV-infected midguts. The potential roles of the predicted targets of the miRNAs that interacted with the circRNAs showed that ubiquitin, apoptosis, and endocytosis signaling pathways were enriched significantly by BmNPV infection.