Comprehensive Egg Coat Proteome of the Ascidian Ciona intestinalis Reveals Gamete Recognition Molecules Involved in Self-sterility*
Comprehensive Egg Coat Proteome of the Ascidian Ciona intestinalis Reveals Gamete Recognition Molecules Involved in Self-sterility*
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DOI：
10.1074/jbc.m809672200
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发表时间：
2009-04
影响因子：
4.8
通讯作者：
Lixy Yamada;Takako Saito;H. Taniguchi;H. Sawada;Y. Harada
中科院分区：
文献类型：
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作者：
Lixy Yamada;Takako Saito;H. Taniguchi;H. Sawada;Y. Harada
尽管卵外壳蛋白在配子识别中起着核心作用，但对其功能和组成知之甚少。在这里，我们报告说，蛋白质组的卵外套在孤独的海鞘玻璃海鞘，称为卵黄外套（VC）部分，包含800多个蛋白质鉴定的质谱分析。与不含VC的鸡蛋蛋白质组相比，VC组分中富集了超过100种蛋白质。VC中最丰富的成分是载脂蛋白样蛋白。VC中含有哺乳动物透明带（ZP）蛋白的多个同源物，其数量出乎意料地大，其中大多数具有表皮生长因子样重复序列。此外，本研究揭示了两个纤维蛋白原样蛋白，v-Themis-A和-B，这两个都是在VC中表达，是负责两个自我不育基因座，我们以前的遗传研究，在这个物种。
Despite central roles of egg coat proteins in gamete recognition, their functions and composition are poorly understood. Here, we report that the proteome of the egg coat in the solitary ascidian Ciona intestinalis, called vitelline coat (VC) fraction, contains more than 800 proteins identified by mass spectrometry-based analyses. Over 100 proteins were enriched in the VC fraction compared with the VC-free egg proteome. The most abundant component in the VC was an apolipoprotein-like protein. The VC contained multiple homologs of mammalian zona pellucida (ZP) proteins, the number of which was unexpectedly large and most of which possessed epidermal growth factor-like repeats. Furthermore, the present study revealed that two fibrinogen-like proteins, v-Themis-A and -B, both of which are expressed in the VC, are the molecules responsible for the two self-sterility loci that were identified by our previous genetic study in this species.