Biomimetic Mg-substituted hydroxyapatite: from synthesis to in vivo behaviour
Biomimetic Mg-substituted hydroxyapatite: from synthesis to in vivo behaviour
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DOI：
10.1007/s10856-006-0032-y
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发表时间：
2008-01-01
影响因子：
3.7
通讯作者：
Sprio, Simone
中科院分区：
文献类型：
--
作者：
Landi, Elena;Logroscino, Giandomenico;Sprio, Simone
使用氯化镁、氢氧化钙和磷酸作为反应物，将镁离子（相对于Ca在5-10摩尔%的范围内）掺入到羟基磷灰石结构中，这对于人造骨的开发具有极大的意义。在合成的粉末中，由于其更好的化学-物理特性，选择了含有5.7% Mg替代钙的合成HA粉末，并将其转化为400-600 μ m的颗粒，用于生物相容性试验（遗传毒性、致癌性、毒性、体外细胞毒性和体内皮肤刺激-致敏试验）。在新西兰白色兔上进行体内试验，使用该骨水泥作为股骨缺损的填充物：与作为对照的商业化学计量HA骨水泥相比，发现骨传导性和再吸收增强。
The incorporation of magnesium ions (in the range 5-10 mol% in respect to Ca) into the hydroxyapatite structure, which is of great interest for the developing of artificial bone, was performed using magnesium chloride, calcium hydroxide and phosphoric acid, as reactants. Among the synthesized powders, the synthetic HA powder containing 5.7% Mg substituting for calcium was selected, due to its better chemico-physical features, and transformed into granules of 400-600 mu m, for biocompatibility tests (genotoxicity, carcinogenicity, toxicity, in vitro cytotoxicity and in vivo skin irritation-sensitization tests). In vivo tests were carried out on New Zealand White rabbits using the granulate as filling for a femoral bone defect: osteoconductivity and resorption were found to be enhanced compared to commercial stoichiometric HA granulate, taken as control.