Comparison of rabbit corneal endothelial cell precursors in the central and peripheral cornea
Comparison of rabbit corneal endothelial cell precursors in the central and peripheral cornea
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DOI：
10.1167/iovs.05-0630
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发表时间：
2005-10-01
影响因子：
4.4
通讯作者：
Amano, S
中科院分区：
文献类型：
--
作者：
Mimura, T;Yamagami, S;Amano, S
目的.比较兔角膜内皮前体细胞在角膜中央和周边区域的分布和自我更新能力。将新西兰白色兔角膜的角膜内皮（CE）和后弹力层分为外周区（直径6.0 - 10.0 mm）和中央区（直径6.0 mm）。然后进行成球测定以从每个区域的CE中分离前体。比较了中心区和外周区的初级和次级球集落数量以及初级球的大小。从CE的外周和中心区域分离原代球体。周边区域的初级球体形成率（34.4 +/- 10.4/10，000个细胞）显著高于角膜中央区域（26.8 +/- 6.6/10，000个细胞; P = 0.0042），但两个区域之间的初级球体大小没有显著差异。外周区的自我更新能力高于中心区，外周区细胞的次级球形成率（39.0 ± 8.8/10，000细胞）明显高于中心区细胞（25.4 ± 4.2/10，000细胞; P = 0.00028）。这些结果表明，周边和中央兔角膜上皮细胞含有显着数量的前体，但周边内皮细胞含有更多的前体，并具有较强的自我更新能力比中央区域。
PURPOSE. To compare the distribution and self-renewal capacity of rabbit corneal endothelial cell precursors in the central and peripheral regions of the cornea.METHODS. The corneal endothelium (CE) and Descemet's membrane of New Zealand White rabbit corneas were divided into a peripheral region (6.0 - 10.0 mm in diameter) and a central region ( 6.0 mm in diameter). Then a sphere-forming assay was performed to isolate precursors from the CE of each region. Numbers of primary and secondary sphere colonies and sizes of primary spheres were compared between the central and peripheral regions.RESULTS. Primary spheres were isolated from the peripheral and the central regions of the CE. The rate of primary sphere formation in the peripheral region (34.4 +/- 10.4/10,000 cells) was significantly higher than in the central cornea ( 26.8 +/- 6.6/10,000 cells; P = 0.0042), but there was no significant difference in the size of primary spheres between the two regions. Self-renewal capacity was higher in the peripheral region than in the central region, as evidenced by a significantly higher secondary sphere formation rate for cells from the periphery (39.0 +/- 8.8/10,000 cells) compared with that for cells from the central region (25.4 +/- 4.2/10,000 cells; P = 0.00028).CONCLUSIONS. These findings demonstrate that peripheral and central rabbit corneal epithelia contain a significant number of precursors but that the peripheral endothelium contains more precursors and has a stronger self-renewal capacity than the central region.